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. 2020 Oct 5:53:e20200032.
doi: 10.1590/0037-8682-0032-2020. eCollection 2020.

Antibacterial activity of Cinnamomum cassia L. essential oil in a carbapenem- and polymyxin-resistant Klebsiella aerogenes strain

Nathalie Gaebler Vasconcelos  1   2 , Késia Esther Silva  1 , Júlio Croda  3   4 , Simone Simionatto  1
Affiliations

Antibacterial activity of Cinnamomum cassia L. essential oil in a carbapenem- and polymyxin-resistant Klebsiella aerogenes strain

Nathalie Gaebler Vasconcelos et al. Rev Soc Bras Med Trop. .

Abstract

Introduction: Essential oils can serve as novel sources of antibiotics for multidrug-resistant bacteria.

Methods: The multidrug-resistance profile of a Klebsiella aerogenes strain was assessed by PCR and sequencing. The antibacterial activity of Cinnamomum cassia essential oil (CCeo) against K. aerogenes was assessed by broth microdilution and time-kill methods.

Results: K. aerogenes showed high antibiotic resistance. The genes bla KPC-2, ampC, bla CTX-M-15, bla OXA-1, and bla TEM were present. CCeo exhibited an inhibitory effect with a minimum inhibitory concentration of 17.57 μg/mL.

Conclusions: The antibacterial activity of CCeo makes it a potential candidate for treating carbapenem- and polymyxin-resistant K. aerogenes strains.

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Conflict of interest statement

Conflict of Interest: The authors declare no conflicts of interest.

Figures

FIGURE 1:
FIGURE 1:. The chromatographic profile obtained with high-resolution gas chromatography for Cinnamomum cassia oil using an AGILENT 7820A Gas Chromatograph. Column: HP-5 30 m × 0.32 mm × 0.25 μm (AGILENT). Temp.: Column: 70°C (0 min), 3°C/min at 240°C. Injector: 240°C Split: 1/50. FID detector: 250°C. Vol. injection time: 1 µL (conc 1.0% in chloroform). Vany Ferraz, Chemistry Department, Universidade Federal de Minas Gerais. Requested by Ferquima (Vargem Grande Paulista, SP, Brazil).
FIGURE 2:
FIGURE 2:. Time-kill curves with CCeo against a carbapenem and polymyxin-resistant K. aerogenes strain. CCeo: Cinnamomum cassia L. essential oil; MIC: minimum inhibitory concentration; PMB: polymyxin B; TGC: tigecycline; C+: positive control (tigecycline at twice the MIC value); C-: negative control (K. aerogenes and brain heart infusion broth).
See this image and copyright information in PMC

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