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. 2020 Sep 29:2020:10.17912/micropub.biology.000314.
doi: 10.17912/micropub.biology.000314.

C. elegans CYLC-2 localizes to sperm

Amber R Krauchunas  1 , Michael Werner  2 , Nicholas Britt  2 , Dawn S Chen  1   3 , Amy S Maddox  2 , Andrew Singson  1
Affiliations

C. elegans CYLC-2 localizes to sperm

Amber R Krauchunas et al. MicroPubl Biol. .
No abstract available

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Figures

Figure 1. CYLC-2::mNeonGreen localizes to sperm and has no effect on sperm function
Figure 1. CYLC-2::mNeonGreen localizes to sperm and has no effect on sperm function
DIC and fluorescence images of young adult hermaphrodites (A-B), males (C-D), and dissected spermatids (E-F). Fluorescence signal clearly labels sperm in and near the spermatheca in the hermaphrodite and spermatids in the male. Scale bar, 50 µm (A-D). Scale bar, 5 µm (E-F). When AD294 males mate with N2 hermaphrodites, the CYLC-2::mNG labeled male sperm are visible in the hermaphrodite reproductive tract (G). Scale bar, 50 µm. Total outcross progeny (H) and percent outcross progeny (I) are comparable between control him-5 males and cylc-2::mNG; him-5 males indicating that tagging CYLC-2 with mNG has no effect on sperm formation or function. Median and 95% confidence intervals are shown in the graphs.
See this image and copyright information in PMC

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