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Review
. 2020 Dec;20(23):e1900368.
doi: 10.1002/pmic.201900368. Epub 2020 Oct 26.

DNA-Barcoded Fluorescence Microscopy for Spatial Omics

Florian Schueder  1   2 , Eduard M Unterauer  1   2 , Mahipal Ganji  1   2 , Ralf Jungmann  1   2
Affiliations
Review

DNA-Barcoded Fluorescence Microscopy for Spatial Omics

Florian Schueder et al. Proteomics. 2020 Dec.

Abstract

Innovation in genomics, transcriptomics, and proteomics research has created a plethora of state-of-the-art techniques such as nucleic acid sequencing and mass-spectrometry-based proteomics with paramount impact in the life sciences. While current approaches yield quantitative abundance analysis of biomolecules on an almost routine basis, coupling this high content to spatial information in a single cell and tissue context is challenging. Here, current implementations of spatial omics are discussed and recent developments in the field of DNA-barcoded fluorescence microscopy are reviewed. Light is shed on the potential of DNA-based imaging techniques to provide a comprehensive toolbox for spatial genomics and transcriptomics and discuss current challenges, which need to be overcome on the way to spatial proteomics using high-resolution fluorescence microscopy.

Keywords: DNA nanotechnology; fluorescence microscopy; high-content imaging; multiplexing; super-resolution microscopy.

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