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. 2020 Nov:85:104589.
doi: 10.1016/j.meegid.2020.104589. Epub 2020 Oct 8.

Isolation, genetic and immunohistochemical identification of Toxoplasma gondii from human placenta in a large toxoplasmosis outbreak in southern Brazil, 2018

Fernanda Pinto-Ferreira  1 , Beatriz de Souza Lima Nino  2 , Felippe Danyel Cardoso Martins  3 , Thais Cabral Monica  3 , Isadora Cortella Britto  3 , Ariana Signori  3 , Kerlei Cristina Medici  4 , Roberta Lemos Freire  5 , Italmar Teodorico Navarro  6 , João Luis Garcia  7 , Selwyn Arlington Headley  8 , Fernanda Silveira Flores Vogel  9 , Camila E Minuzzi  9 , Luiza Pires Portella  9 , Patricia Bräunig  9 , Luis Antonio Sangioni  9 , Aline Ludwig  9 , Luciane Silva Ramos  10 , Liliane Pacheco  10 , Camila Ribeiro Silva  11 , Flávia Caselli Pacheco  12 , Ivone Andreatta Menegolla  12 , Lourdes Bonfleur Farinha  13 , Simone Haas  14 , Natalia Canal  15 , José Roberto Mineo  16 , Cledison Marcio Difante  17 , Regina Mitsuka-Breganó  18
Affiliations

Isolation, genetic and immunohistochemical identification of Toxoplasma gondii from human placenta in a large toxoplasmosis outbreak in southern Brazil, 2018

Fernanda Pinto-Ferreira et al. Infect Genet Evol. 2020 Nov.

Abstract

The present study aimed to describe a molecular analysis of environmental and pork samples, the isolation, genetic identification and immunohistochemistry (IHC) of Toxoplama gondii from placenta and amniotic fluid from five pregnant women that miscarried during a toxoplasmosis outbreak in 2018, Santa Maria, Rio Grande do Sul. Environmental and pork samples were submitted to polymerase chain reaction (PCR); placenta and amniotic fluid samples to histopathology, IHC, mouse bioassay and PCR. All samples were genotyped by PCR-RFLP with 11 loci. Histopathologic and IHC were compatibles with toxoplasmosis. All pregnants were positive in PCR and bioassay, the genotypes were compared, and all were equal suggesting a same source of infection. Among the environmental and food samples, a sludge sample from a water tank and two porks samples were positive in PCR, and the genotypes were different from the pregnant women isolates. It is concluded that obtain and compare isolates is essential to elucidate outbreak source.

Keywords: Bioassay; Environment; Pregnant woman; Santa Maria.

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