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Randomized Controlled Trial
. 2021 May;76(5):1385-1397.
doi: 10.1111/all.14621. Epub 2020 Oct 29.

Longitudinal egg-specific regulatory T- and B-cell development: Insights from primary prevention clinical trials examining the timing of egg introduction

Catherine L Lai  1   2   3   4 , Dianne E Campbell  1   2   3   4 , Debra J Palmer  4   5   6 , Maria Makrides  7   8 , Brigitte Santner-Nanan  9 , Michael Gold  10 , John Wei-Liang Tan  1 , Carol Valerio  1 , Ralph Nanan  9 , Susan L Prescott  4   11   12 , Peter S Hsu  1   2   3   4
Affiliations
Randomized Controlled Trial

Longitudinal egg-specific regulatory T- and B-cell development: Insights from primary prevention clinical trials examining the timing of egg introduction

Catherine L Lai et al. Allergy. 2021 May.

Abstract

Background: Egg allergy affects almost 1 in 10 Australian infants. Early egg introduction has been associated with a reduced risk in developing egg allergy; however, the immune mechanisms underlying this protection remain unclear.

Objective: To examine the role of regulatory immune cells in tolerance induction during early egg introduction.

Methods: Cryopreserved peripheral blood mononuclear cells (PBMC) were obtained from infants from 2 randomized controlled trials of early introduction of egg for the primary prevention of egg allergy; BEAT (at 12 months, n = 42) and STEP (at 5 months n = 82; 12 months n = 82) study cohorts. In vitro ovalbumin-stimulated PBMC were analyzed by flow cytometry for presence of ovalbumin-specific regulatory T cells, using activation markers, FoxP3, and IL-10 expression. Ovalbumin-specific regulatory B cells were identified by co-expression of fluorescence-conjugated ovalbumin and IL-10.

Results: Specific, age-dependent expansion of ovalbumin-specific regulatory T cells was only observed in infants who (a) had early egg introduction and (b) did not have egg allergy at 12 months. This expansion was blunted or impaired in children who did not undergo early egg introduction and in those with clinical egg allergy at 12 months. Infants with egg allergy at 12 months of age also had reduced frequency of ovalbumin-specific regulatory B cells compared to egg-tolerant infants.

Conclusion: Early egg introduction and clinical tolerance to egg were associated with expansion of ovalbumin-specific T and B regulatory cells, which may be an important developmental process for tolerance acquisition to food allergens.

Keywords: egg allergy; ovalbumin; regulatory B cell; regulatory T cell; tolerance.

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References

REFERENCES

    1. Osborne NJ, Koplin JJ, Martin PE, et al. Prevalence of challenge-proven IgE-mediated food allergy using population-based sampling and predetermined challenge criteria in infants. J Allergy Clin Immunol. 2011;127(3):668-676.
    1. Tang ML, Mullins RJ. Food allergy: is prevalence increasing? Intern Med J. 2017;47(3):256-261.
    1. Du Toit G, Roberts G, Sayre PH, et al. Randomized trial of peanut consumption in infants at risk for peanut allergy. N Engl J Med. 2015;372(9):803-813.
    1. Wei-Liang Tan J, Valerio C, Barnes EH, et al. A randomized trial of egg introduction from 4 months of age in infants at risk for egg allergy. J Allergy Clin Immunol. 2017;139(5):1621-1628.
    1. Palmer DJ, Sullivan TR, Gold MS, Prescott SL, Makrides M. Randomized controlled trial of early regular egg intake to prevent egg allergy. J Allergy Clin Immunol. 2017;139(5):1600-1607.

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