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. 2021 Mar;19(3):409-411.
doi: 10.1111/pbi.13489. Epub 2020 Nov 14.

Tissue-specific activation of DOF11 promotes rice resistance to sheath blight disease and increases grain weight via activation of SWEET14

Pyol Kim  1 , Cai Yun Xue  2 , Hyon Dok Song  1 , Yue Gao  2 , Lu Feng  2 , Yuhua Li  1 , Yuan Hu Xuan  2
Affiliations

Tissue-specific activation of DOF11 promotes rice resistance to sheath blight disease and increases grain weight via activation of SWEET14

Pyol Kim et al. Plant Biotechnol J. 2021 Mar.
No abstract available

Keywords: DOF11; SWEET14; rice; sheath blight disease; yield.

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Figures

Figure 1
Figure 1
DOF11 activates SWEET14 to regulate rice production and resistance to ShB. (a) PBZ1 and SWEET14 expression levels were examined after 0, 24, 48 and 72 h of R. solani inoculation using qRT‐PCR. Data indicate the average ± SE (n = 3). (b) Genomic structure and mutant information of SWEET14. Black boxes and lines indicate exons and introns, respectively. The sequences below the fifth exon indicate wild‐type (WT) and CRISPR/Cas9‐induced genome editing mutant sequences. (c) Expression of SWEET14 in WT, sweet14 (#1, #2) and SWEET14 OX (#1, #2) was examined. Data indicate the average ± SE (n = 3). (d) Leaves from the WT, sweet14 (#1, #2) and SWEET14 OX (#1, #2) were inoculated with R. solani. The lesion areas on the leaf surfaces were examined. Data indicate the average ± SE (n > 10). (e) The model of SWEET14 action in rice defence to R. solani. (f) Three‐month‐old WT, empty vector (EV), sweet14 (#1, #2) and SWEET14 OX (#1, #2) plants. (g) One‐thousand‐grain weight, (h) number of tillers per plant and (i) number of grains per panicle from WT, sweet14 (#1, #2) and SWEET14 OX (#1, #2). Data indicate the average ± SE (n > 10). (j) The library for yeast one‐hybrid was constructed in pGAD42, and 2‐kb of SWEET14 promoter was cloned into pHISi vector with HIS as the reporter gene. The yeast cells co‐transformed with pSWEET14‐His and library DNA were grown on SD media (‐Try, ‐Leu and –His) or the same medium containing 5 mM 3‐amino‐1,2,4‐triazole (3AT), a competitive inhibitor of HIS3. (k) Genomic structure and mutant information of dof11. Black boxes and lines indicate exons and introns, respectively. The sequences below the first exon indicate WT and CRISPR/Cas9‐induced genome editing mutant. (l) Leaves from the WT and dof11 were inoculated with R. solani AG1‐IA. The lesion areas on the leaf surfaces were examined. Data indicate the average ± SE (n > 10). (m) The expression of DOF11 in WT and DOF11 OX (#1‐#4) was examined using qRT‐PCR. Data indicate the average ± SE (n = 3). (n) Three‐month‐old WT and DOF11 OX (#1‐#2) plants were photographed. (o) Leaves from the WT and DOF11 OX (#1, #2) at the time of inoculated with R. solani and after infection. The lesion areas on the leaf surfaces were examined. Data indicate the average ± SE (n > 10). (p) The number of tillers per plant, (q) number of grains per panicle, (r) one‐thousand‐grain weight from WT and DOF11 OX (#1, #2). Data indicate the average ± SE (n > 10). (s) DOF11‐GFP and DOF11‐VP16‐GFP localization in the protoplast cells. GFP and bright‐field channels were evaluated. Scale bar = 20 µm. (t) Three‐month‐old WT, dof11 and dof11/DOF11‐VP16‐Myc (#1, #2). (u) Western blot analysis was performed to detect DOF11‐VP16‐Myc levels using anti‐Myc antibody in WT, dof11 and dof11/DOF11‐VP16‐Myc plants (#1, #2). Coomassie brilliant blue (CBB) staining was used as the loading control. (v) Leaves from the WT and dof11/DOF11‐VP16‐Myc were inoculated with R. solani. (q) The lesion areas on the leaf surfaces were examined. Data indicate the average ± SE (n > 10). (w) One‐thousand‐grain weight, (x) number of tillers per plant, and (y) number of grains per panicle from WT, dof11, and dof11/DOF11‐VP16‐Myc (#1, #2). Data indicate the average ± SE (n> 10). (z) Schematic diagram indicating the location of the probes (P1‐P5) used for chromatin immunoprecipitation (ChIP) assays in SWEET14 promoter. Relative ratios of immunoprecipitated DNA to input DNA were determined by qPCR. Input DNA was used to normalize the data. −Ab or +Ab: Myc antibody. Error bars represent ± SE (n = 3). (o) One‐thousand‐grain weight of WT, dof11 and dof11/DOF11‐VP16‐Myc (#1, #2). Data indicate the average ± standard error (SE) (n > 10). (ii) SWEET14 expression was examined in the root, leaf, mesophyll cells (MC), root hairs (RH) and mature booting stage (MB) from WT, DOF11 OX1 and dof11/DOF11‐VP16‐Myc #1 plants using qRT‐PCR. Data indicate the average ± SE (n = 3). (iii) One‐thousand‐grain weight of WT, sweet14, DOF11‐VP16 and sweet14/DOF11‐VP16 plants. Data indicate the average ± SE (n > 10). (iv) Leaves from the WT, sweet14, DOF11‐VP16 and sweet14/DOF11‐VP16 plants were inoculated with R. solani. The lesion areas on the leaf surfaces were examined. Data indicate the average ± SE (n > 10). Different letters above the bars denote statistically significant differences (P < 0.05).
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