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. 2020 Dec;49(8):677-685.
doi: 10.1007/s00249-020-01468-3. Epub 2020 Oct 14.

On the utility of fluorescence-detection analytical ultracentrifugation in probing biomolecular interactions in complex solutions: a case study in milk

Jennifer M Crowther  1   2 , Marita Broadhurst  3 , Thomas M Laue  4 , Geoffrey B Jameson  5   6   7 , Alison J Hodgkinson  8   9 , Renwick C J Dobson  10   11   12
Affiliations

On the utility of fluorescence-detection analytical ultracentrifugation in probing biomolecular interactions in complex solutions: a case study in milk

Jennifer M Crowther et al. Eur Biophys J. 2020 Dec.

Abstract

β-Lactoglobulin is the most abundant protein in the whey fraction of ruminant milks, yet is absent in human milk. It has been studied intensively due to its impact on the processing and allergenic properties of ruminant milk products. However, the physiological function of β-lactoglobulin remains unclear. Using the fluorescence-detection system within the analytical ultracentrifuge, we observed an interaction involving fluorescently labelled β-lactoglobulin in its native environment, i.e. cow and goat milk, for the first time. Co-elution experiments support that these β-lactoglobulin interactions occur naturally in milk and provide evidence that the interacting partners are immunoglobulins, while further sedimentation velocity experiments confirm that an interaction occurs between these molecules. The identification of these interactions, made possible through the use of fluorescence-detected analytical ultracentrifugation, provides possible clues to the long debated physiological function of this abundant milk protein.

Keywords: Analytical ultracentrifugation; Fluorescence; Immunoglobulin; Protein interactions; β-Lactoglobulin.

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