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. 2021 Feb;28(7):8266-8280.
doi: 10.1007/s11356-020-11021-6. Epub 2020 Oct 14.

Genotoxic and epigenetic effects of diuron in the Pacific oyster: in vitro evidence of interaction between DNA damage and DNA methylation

Farida Akcha  1 , Audrey Barranger  2 , Evelyne Bachère  3
Affiliations

Genotoxic and epigenetic effects of diuron in the Pacific oyster: in vitro evidence of interaction between DNA damage and DNA methylation

Farida Akcha et al. Environ Sci Pollut Res Int. 2021 Feb.

Abstract

Recently, research has contributed to better knowledge on the occurrence of pesticides in coastal water by identifying frequently detected substances, their concentration range and their acute and chronic toxicity for organisms. Pesticide pollution is of particular concern in France due to important agricultural activities and presence of several exoreic catchment areas that vehicle pesticides up to coastal waters, impacting non-target marine species. Several ecotoxicology questions remain to be addressed concerning the long-term effects of chronic pesticide exposure and the mechanisms involved in adaptation to chemical stress. In the present study, we brought new insights on the genetic and epigenetic effects of the herbicide diuron in oyster genitors. During gametogenesis, we exposed Crassostrea gigas to environmentally realistic herbicide concentrations (0.2-0.3 μg L-1 during two 7-day periods at half-course and end of gametogenesis). Diuron exposure was shown to decrease global DNA methylation and total methyltransferase activity in whole oyster tissue; this is consistent with the previous observation of a significant decrease in DNMT1 gene expression. Diuron effect seemed to be tissue-specific; hypermethylation was detected in the digestive gland, whereas diuron exposure had no effect on gill and gonad tissue. The genotoxicity of diuron was confirmed by the detection of one adduct in gonad DNA. By using in vitro approaches and human DNMT1 (DNMT1 has not been purified yet in bivalves), the presence of DNA lesions (adduct, 8-oxodGuo) was shown to interfere with DNMT1 activity, indicating a complex interaction between DNA damage and DNA methylation. Based on our results, we propose mechanisms to explain the effect of diuron exposure on DNA methylation, a widespread epigenetic mark.

Keywords: 8-oxodGuo; DNA adducts; DNA damage; DNA methylation; Diuron; Genotoxicity; Oyster; Pesticide.

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References

    1. Ahmad I, Ahmad A, Ahmad M (2016) Binding properties of pendimethalin herbicide to DNA: multispectroscopic and molecular docking approaches. Phys Chem Chem Phys 18:6476–6485. https://doi.org/10.1039/c5cp07351k - DOI
    1. Akcha F, Spagnol C, Rouxel J (2012) Genotoxicity of diuron and glyphosate in oyster spermatozoa and embryos. Aquat Toxicol 106:104–113. https://doi.org/10.1016/j.aquatox.2011.10.018 - DOI
    1. Akcha F, Barranger A, Bachere E, Berthelin CH, Piquemal D, Alonso P, Sallan RR, Dimastrogiovanni G, Porte C, Menard D, Szczybelski A, Benabdelmouna A, Auffret M, Rouxel J, Burgeot T (2016) Effects of an environmentally relevant concentration of diuron on oyster genitors during gametogenesis: responses of early molecular and cellular markers and physiological impacts. Environ Sci Pollut Res Int 23:8008–8020. https://doi.org/10.1007/s11356-015-5969-2 - DOI
    1. Baccarelli A, Bollati V (2009) Epigenetics and environmental chemicals. Curr Opin Pediatr 21:243–251. https://doi.org/10.1097/MOP.0b013e32832925cc - DOI
    1. Bachère E, Barranger A, Bruno R, Rouxel J, Menard D, Piquemal D, Akcha F (2017) Parental diuron-exposure alters offspring transcriptome and fitness in Pacific oyster Crassostrea gigas. Ecotox Environ Safe 142:51–58. https://doi.org/10.1016/j.ecoenv.2017.03.030 - DOI

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