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. 2020 Oct 12:15:111.
doi: 10.1186/s13020-020-00391-1. eCollection 2020.

Ganoderma spore powder contains little triterpenoids

Mei-Ting Liu #  1 , Ling-Xiao Chen #  1 , Jing Zhao  1 , Shao-Ping Li  1
Affiliations

Ganoderma spore powder contains little triterpenoids

Mei-Ting Liu et al. Chin Med. .

Abstract

Background: Ganoderma spore is a minuscule germ cell ejected from Ganoderma gills during its growth maturity period, it has been considered with high exploitable potential in health-care products manufacture.

Methods: After testing sporoderm-broken rate, the triterpenoids in 12 batches of broken and unbroken Ganoderma spore powder (GSP) samples were compared with Ganoderma lucidum fruiting body (GL) by high performance thin-layer chromatography (HPTLC) and further verified by liquid chromatography coupled with electrospray ionization quadrupole-time-of-flight mass spectrometry (LC-QTOF-MS); meanwhile, the dissolution of triterpenoids after bionic extraction was also investigated by HPTLC.

Results: The sporoderm-broken rate of all the broken GSP samples was over 85%. The relative peak area of triterpenoids in GSP samples were lower than 50% of that in fruiting body, and the dissolution of triterpenoids in artificial gastrointestinal fluid was lower than in methanol.

Conclusions: This study demonstrated that there were little triterpenoids in GSP. Triterpenoids in GSP also seldom be dissolved in artificial gastrointestinal fluid.

Keywords: Artificial gastroenteric fluid; Bionic extraction; Ganoderma spore powder; HPTLC; LC–QTOF–MS; Triterpenoids.

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Conflict of interest statement

Competing interestsThe authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
HPTLC fingerprint chromatogram of GSP under UV 365 nm (a). Grayscale scanning integral results of GL and GSP-05 (b)
Fig. 2
Fig. 2
Three major fragment ions of oxygenated tetracyclic triterpenoid (a). Possible structures of P1, P2 and P3 (b). Extraction ion chromatograms (MS2) of GL and GSP-05 (c)
Fig. 3
Fig. 3
HPTLC chromatogram of GSP in artificial gastric fluid (a). HPTLC chromatogram of GSP in artificial intestinal fluid (b). HPTLC chromatogram of GSP after bionic extraction (c). B1—The blank of artificial gastric fluid; B2—The blank of artificial intestinal fluid
See this image and copyright information in PMC

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