Effects of natural sunlight on antimicrobial-resistant bacteria (AMRB) and antimicrobial-susceptible bacteria (AMSB) in wastewater and river water

Abstract

The effects of natural sunlight on antimicrobial-resistant bacteria (AMRB) and antimicrobial-susceptible bacteria (AMSB) were investigated in three types of water: sewage treatment plant (STP) influent, STP secondary effluent, and river water in an urban area of Japan. The AMRB were grouped into six classes: carbapenem-resistant Enterobacteriaceae (CRE), extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae (ESBL-E), multi-drug-resistant Acinetobacter (MDRA), multi-drug-resistant Pseudomonas aeruginosa (MDRP), methicillin-resistant Staphylococcus aureus (MRSA), and vancomycin-resistant Enterococcus (VRE). The amount of each group of bacteria present was estimated using specific chromogenic agar formulations. AMRB were detected in all water samples, with 13-2,407 colony-forming units (CFU)/mL in the STP influent, N.D. to 202 CFU/mL in the secondary STP effluent, and N.D. to 207 CFU/mL in the river water. The distribution profiles of the AMSB in water samples were similar to those of AMRB. The degree to which AMRB and AMSB present in the river water were inactivated by natural sunlight was tested as the main aim of this study. Irradiation by natural sunlight was found to inactivate almost 100% of all the target AMRB after 5 h of exposure, with no significant differences (P < 0.05) observed in the effects that sunlight had on AMSB and AMRB. Analysis of the bacterial community structure based on 16S rRNA gene sequencing showed that the structure of the bacterial community was apparently not affected by the exposure to sunlight. In addition, the taxonomic diversity in the STP secondary effluent did not change as a result of additional disinfection with chlorine. The results of this study suggest that it is possible that exposure to sunlight could be used as an alternative to disinfection via chlorine. To our knowledge, this is the first report to demonstrate the mitigation of AMSB and AMRB pollution in a river environment via the exposure to natural sunlight.

Keywords: Antimicrobial-resistant bacteria (AMRB); Antimicrobial-susceptible bacteria (AMSB); Hospital effluent; Natural light; River environment; Sewage treatment plant (STP).