Physiological and molecular responses in halotolerant Dunaliella salina exposed to molybdenum disulfide nanoparticles

Abstract

Molybdenum disulfide nanoparticles (MoS₂ NPs) has emerged as the promising nanomaterial with a wide array of applications in the biomedical, industrial and environmental field. However, the potential effect of MoS₂ NPs on marine organisms has yet to be reported. In this study, the effect of MoS₂ NPs on the physiological index, subcellular morphology, transcriptomic profiles of the marine microalgae Dunaliella salina was investigated for the first time. exhibited "doping-like" effects on marine microalgae; Growth stimulation was 193.55%, and chlorophyll content increased 1.61-fold upon the addition of 50 μg/L MoS₂ NPs. Additionally, exposure to MoS₂ NPs significantly increased the protein and carbohydrate content by 2.03- and 1.56-fold, respectively. The antioxidant system was activated as well to eliminate the adverse influence of reactive oxygen species (ROS). Transcriptomic analysis revealed that genes involved in porphyrin synthesis, glycolysis/gluconeogenesis, tricarboxylic acid cycle and DNA replication were upregulated upon MoS₂ NPs exposure, which supports the mechanistic role of MoS₂ NPs in improving cellular growth and photosynthesis. The "doping-like" effects on marine algae suggest that the low concentration of MoS₂ NPs might change the rudimentary ecological composition in the ocean.

Keywords: Marine algae; MoS(2) NPs; Oxidative stress; Transcriptomic analysis; “Doping-like” effects.