Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1987;24(4):37-42.

[Cultivation of cells in a medium with blood hydrolysate]

[Article in Bulgarian]
E Veleva, V Katevska, P Tekerlekov, P Gergov, P Krŭstev
  • PMID: 3307135
Comparative Study

[Cultivation of cells in a medium with blood hydrolysate]

[Article in Bulgarian]
E Veleva et al. Vet Med Nauki. 1987.

Abstract

Use was made of fresh blood of swine, diluted with an equal amount of distilled water to produce a protein hydrolysate. Enzyme hydrolysis was effected through extracellular alkaline protease produced by stain DI of Bacillus subtilis. The cell lines BHK-21, PK-15 and spzv were cultured in a nutrient medium containing 0.180-0.200 mg/cm3 alpha-amine nitrogen and relevant growth factors of nonprotein character. It was found that the cells cultured in this medium showed no differences with regard both to morphology and karyology as against the control cells which were treated with the classic medium of Eagle. It was also found that the medium could successfully be used instead of the imported nutrient media.

PubMed Disclaimer