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. 2020 Sep 24:11:576680.
doi: 10.3389/fmicb.2020.576680. eCollection 2020.

Degradation and Toxicity Analysis of a Reactive Textile Diazo Dye-Direct Red 81 by Newly Isolated Bacillus sp. DMS2

Shivani Amin  1 , Rajesh Prasad Rastogi  1 , Mukesh Ghanshyam Chaubey  2 , Kunal Jain  1 , Jyoti Divecha  3 , Chirayu Desai  4 , Datta Madamwar  1   4
Affiliations

Degradation and Toxicity Analysis of a Reactive Textile Diazo Dye-Direct Red 81 by Newly Isolated Bacillus sp. DMS2

Shivani Amin et al. Front Microbiol. .

Abstract

An efficient diazo dye degrading bacterial strain, Bacillus sp. DMS2 was isolated from a long-term textile dye polluted environment. The strain was assessed for its innate ability to completely degrade and detoxify Direct Red 81 (DR81) textile dye under microaerophilic conditions. The degradation ability of strain showed significant results on optimizing the nutritional and environmental parameters. Based on statistical models, maximum efficiency of decolorization achieved within 24 h for 100 mg/l of dye supplemented with glucose (0.02%), MgSO4 (0.002%) and urea (0.5%) at 30°C and pH (7.0). Moreover, a significant catabolic induction of a laccase and azoreductase suggested its vital role in degrading DR81 into three distinct metabolites (intermediates) as by-products. Further, toxicity analysis of intermediates were performed using seeds of common edible plants, aquatic plant (phytotoxicity) and the nematode model (animal toxicity), which confirmed the non-toxic nature of intermediates. Thus, the inclusive study of DMS2 showed promising efficiency in bioremediation approach for treating industrial effluents.

Keywords: Caenorhabditis elegans; Direct Red 81; Lemna minor; biodegradation; response surface methodology; toxicity.

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Figures

FIGURE 1
FIGURE 1
Decolorization efficiency of Bacillus sp. DMS2 toward 30 different azo dyes under optimized conditions.
FIGURE 2
FIGURE 2
Contour plots of the decolorization with different variables: (A) MgSO4 v/s Glucose; (B) temperature v/s pH and (C) MgSO4 v/s urea.
FIGURE 3
FIGURE 3
Effect of initial dye concentration on DMS2 decolorization capacity.
FIGURE 4
FIGURE 4
In vivo ROS production in L. minor seed germination with (A) control sample; (B) dye degraded products and (C) dye.
FIGURE 5
FIGURE 5
Fluorophotometric analysis of in vitro ROS production in L. minor growing with dye and degraded dye products (A) Emission spectra of DCF fluorescence (B) DCF fluorescence intensity.
FIGURE 6
FIGURE 6
(A) Mean survival time of C. elegans with dye (100 mg/l) and metabolites produced after incubation with DMS2, (B) Toxicity analysis on the life span of C. elegans and (C) Effect on pharyngeal pumping.
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