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. 1977 Jul;25(7):899-907.
doi: 10.1177/25.7.330738.

Two-color immunofluorescence using a fluorescence-activated cell sorter

M R Loken, D R Parks, L A Herzenberg

Two-color immunofluorescence using a fluorescence-activated cell sorter

M R Loken et al. J Histochem Cytochem. 1977 Jul.

Abstract

A technique for the analysis of fluorescein and rhodamine in a flow system using a single wavelength of excitation is described. Both optics and electronics are used to discriminate the fluorescein and rhodamine signals. This technique has been used to study the relationship between immunoglobulin M and immunoglobulin D on mouse splenic lymphocytes.

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