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. 2021:2219:231-240.
doi: 10.1007/978-1-0716-0974-3_14.

Transcriptomic Analysis in the Sea Anemone Nematostella vectensis

Jacob F Warner  1   2 , Eric Röttinger  3
Affiliations

Transcriptomic Analysis in the Sea Anemone Nematostella vectensis

Jacob F Warner et al. Methods Mol Biol. 2021.

Abstract

The sea anemone Nematostella vectensis is an emerging research model to study embryonic development and regeneration at the molecular and global transcriptomic level. Transcriptomics analysis is now routinely used to detect differential expression at the genome level. Here we present the latest procedures for isolating high-quality RNA required for next generation sequencing, as well as methods and resources for quantifying transcriptomic data.

Keywords: Cnidarian; De novo assembly; Nematostella vectensis; RNA isolation; transcriptome.

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References

    1. Hand C, Uhlinger KR (1992) The culture, sexual and asexual reproduction, and growth of the sea anemone Nematostella vectensis. Biol Bull 182(2):169–176 - PubMed
    1. Reitzel A, Burton P, Krone C, Finnerty J (2007) Comparison of developmental trajectories in the starlet sea anemone Nematostella vectensis: embryogenesis, regeneration, and two forms of asexual fission. Invertebr Biol 126(2):99–112
    1. Passamaneck YJ, Martindale MQ (2012) Cell proliferation is necessary for the regeneration of oral structures in the anthozoan cnidarian Nematostella vectensis. BMC Dev Biol 12(1):1–1
    1. Bossert PE, Dunn MP, Thomsen GH (2013) A staging system for the regeneration of a polyp from the aboral physa of the anthozoan cnidarian Nematostella vectensis. Dev Dyn 242:1320–1331 - PubMed - PMC
    1. Amiel AR, Johnston HT, Nedoncelle K, Warner JF, Ferreira S, Röttinger E (2015) Characterization of morphological and cellular events underlying oral regeneration in the sea anemone, Nematostella vectensis. Int J Mol Sci 16(12):28449–28471 - PubMed - PMC

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