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. 1987 Aug;31(8):1271-3.
doi: 10.1128/AAC.31.8.1271.

Improved sensitivity in assays for binding of novel beta-lactam antibiotics to penicillin-binding proteins of Escherichia coli

K Bush, S A Smith, S Ohringer, S K Tanaka, D P Bonner

Improved sensitivity in assays for binding of novel beta-lactam antibiotics to penicillin-binding proteins of Escherichia coli

K Bush et al. Antimicrob Agents Chemother. 1987 Aug.

Abstract

Tigemonam and temocillin, but not aztreonam, bound to penicillin-binding proteins (PBPs) 1a and 3 of Escherichia coli with apparent improved affinity when challenged with benzylpenicillin at lowered temperatures. Half times for deacylation of the tigemonam-PBP complexes were shorter than were those of the corresponding aztreonam-PBP complexes. The implications of the routine testing of PBP affinities are discussed.

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