Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Oct 19;20(1):316.
doi: 10.1186/s12906-020-03100-5.

Isolation, characterization and in vitro anti-salmonellal activity of compounds from stem bark extract of Canarium schweinfurthii

Jean Baptiste Sokoudjou  1   2 , Olubunmi Atolani  2   3 , Guy Sedar Singor Njateng  1 , Afsar Khan  2 , Cyrille Ngoufack Tagousop  4 , André Nehemie Bitombo  2   5 , Norbert Kodjio  1 , Donatien Gatsing  6
Affiliations

Isolation, characterization and in vitro anti-salmonellal activity of compounds from stem bark extract of Canarium schweinfurthii

Jean Baptiste Sokoudjou et al. BMC Complement Med Ther. .

Abstract

Background: Bacteria belonging to the Salmonella genus are major concern for health, as they are widely reported in many cases of food poisoning. The use of antibiotics remains a main stream control strategy for avian salmonellosis as well as typhoid and paratyphoid fevers in humans. Due to the growing awareness about drug resistance and toxicities, the use of antibiotics is being discouraged in many countries whilst advocating potent benign alternatives such as phyto-based medicine. The objective of this work was to isolate, characterise the bioactive compounds of Canarium schweinfurthii; and evaluate their anti-salmonellal activity.

Methods: The hydro-ethanolic extract of Canarium schweinfurthii was fractionated and tested for their anti-salmonellal activity. The most active fractions (i.e. chloroform and ethyl acetate partition fractions) were then explored for their phytochemical constituents. Fractionation on normal phase silica gel column chromatography and size exclusion chromatography on Sephadex LH-20 led to the isolation of four compounds (maniladiol, scopoletin, ethyl gallate and gallic acid) reported for the first time in Canarium schweinfurthii.

Results: Result indicated that scopoletin and gallic acid had greater activity than the crude extracts and partition fractions. Among the isolated compounds, scopoletin showed the highest inhibitory activity with a MIC of 16 μg/ml against Salmonella Typhimurium and Salmonella Enteritidis.

Conclusions: The overall results of this study indicates that the hydro-ethanolic extract as well as some of isolated compounds have interesting anti-salmonellal activities that could be further explored for the development of potent therapy for salmonellosis. Furthermore, the study adds credence to the folkloric applications of the plant.

Keywords: Canarium schweinfurthii; Ethnomedicine; Natural substances; Salmonellosis.

PubMed Disclaimer

Conflict of interest statement

Authors have declared that no competing interests exist.

Figures

Fig. 1
Fig. 1
Flow chart for the isolation of compounds from the hydroethanolic extract of Canarium schweinfurthii
Fig. 2
Fig. 2
Chemical structures of isolated compounds from Canarium schweinfurthii stem barks extract
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Rajagopal R, Mini M. Outbreaks of salmonellosis in three different poultry farms of Kerala, India. Asian Pac J Trop Biomed. 2013;3(6):496–500. doi: 10.1016/S2221-1691(13)60103-3. - DOI - PMC - PubMed
    1. Awa DN, Achukwi MD. Livestock pathology in the central African region: some epidemiological considerations and control strategies. Anim Health Res Rev. 2010;11:235–244. doi: 10.1017/S1466252309990077. - DOI - PubMed
    1. Ali MZ, Sultana S. Avian salmonellosis, Newcastle disease and aspergillosis. Tech Rep. 2012;25. 10.13140/RG.2.1.1981.0404.
    1. Threlfall EJ, Wain J, Peters T, Lane C, De Pinna E, Little CL, et al. Egg-borne infections of humans with Salmonella: not only an S. Enteritidis problem. Worlds Poult Sci J. 2014;70:15–26. doi: 10.1017/S0043933914000026. - DOI
    1. Carli KT, Unal CB, Caner V, Eyigor A. Detection of Salmonellae in chicken feces by a combination of tetrathionate broth enrichment, capillary PCR, and capillary gel electrophoresis. J Clin Microbiol. 2001;39:1871–1876. doi: 10.1128/JCM.39.5.1871-1876.2001. - DOI - PMC - PubMed