Comparative Study
doi: 10.1016/0167-4781(87)90098-4.
Cloning and sequencing of a carp beta s-crystallin cDNA
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- PMID: 3307924
- DOI: 10.1016/0167-4781(87)90098-4
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Comparative Study
Cloning and sequencing of a carp beta s-crystallin cDNA
Biochim Biophys Acta.
.
Abstract
The mRNAs were extracted from common carp (Cyprinus carpio) lenses, purified, reverse transcribed, dC tailed and cloned into Escherichia coli with pBR322 as vector. The cloning efficiency was around 1 X 10(7) colonies per micrograms of mRNA. A clone (pC20) was found by hybrid-arrested to contain the cDNA related to carp crystallins. However, comparison of the derived amino-acid sequence with bovine gamma-II and beta s-crystallins indicates that this carp crystallin sequence resembles closely the bovine beta s-crystallin and should be better classified as such except that this fish sequence does not contain the N-terminal 'arm' of four amino-acid residues present in bovine beta s-crystallin.
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