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Review
. 2021 Jan 2;12(1):125-144.
doi: 10.1080/21645698.2020.1831729.

Engineering disease resistant plants through CRISPR-Cas9 technology

Swati Tyagi  1 , Robin Kumar  2   3 , Vivak Kumar  3 , So Youn Won  1 , Pratyoosh Shukla  4
Affiliations
Review

Engineering disease resistant plants through CRISPR-Cas9 technology

Swati Tyagi et al. GM Crops Food. .

Abstract

Plants are susceptible to phytopathogens, including bacteria, fungi, and viruses, which cause colossal financial shortfalls (pre- and post-harvest) and threaten global food safety. To combat with these phytopathogens, plant possesses two-layer of defense in the form of PAMP-triggered immunity (PTI), or Effectors-triggered immunity (ETI). The understanding of plant-molecular interactions and revolution of high-throughput molecular techniques have opened the door for innovations in developing pathogen-resistant plants. In this context, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR-associated protein 9 (Cas9) has transformed genome editing (GE) technology and being harnessed for altering the traits. Here we have summarized the complexities of plant immune system and the use of CRISPR-Cas9 to edit the various components of plant immune system to acquire long-lasting resistance in plants against phytopathogens. This review also sheds the light on the limitations of CRISPR-Cas9 system, regulation of CRISPR-Cas9 edited crops and future prospective of this technology.

Keywords: CRISPR-Cas9; Gene editing; biotic stress; disease Resistance; plant immune system; susceptible genes.

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Figures

Figure 1.
Figure 1.
Graphical repersentation of plant defense system
Figure 2.
Figure 2.
Graphical repersentation how CRISPR-Cas9 system work as bacterial immune system
Figure 3.
Figure 3.
General work-flow of developing disease ressistance crops using CRISPR-Cas9 system
Figure 4.
Figure 4.
Regulatory approaches around the globe* over CRISPR edited crops and other GMOs
See this image and copyright information in PMC

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