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Review
. 2020 Oct 16;21(20):7651.
doi: 10.3390/ijms21207651.

Current Status of Circulating Tumor DNA Liquid Biopsy in Pancreatic Cancer

Miles W Grunvald  1 , Richard A Jacobson  1 , Timothy M Kuzel  2 , Sam G Pappas  1 , Ashiq Masood  2   3
Affiliations
Review

Current Status of Circulating Tumor DNA Liquid Biopsy in Pancreatic Cancer

Miles W Grunvald et al. Int J Mol Sci. .

Abstract

Pancreatic cancer is a challenging disease with a low 5-year survival rate. There are areas for improvement in the tools used for screening, diagnosis, prognosis, treatment selection, and assessing treatment response. Liquid biopsy, particularly cell free DNA liquid biopsy, has shown promise as an adjunct to our standard care for pancreatic cancer patients, but has not yet been universally adopted into regular use by clinicians. In this publication, we aim to review cfDNA liquid biopsy in pancreatic cancer with an emphasis on current techniques, clinical utility, and areas of active investigation. We feel that researchers and clinicians alike should be familiar with this exciting modality as it gains increasing importance in the care of cancer patients.

Keywords: cell free DNA; circulating tumor DNA; liquid biopsy; pancreatic cancer.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Potential impact of ctDNA-based liquid biopsy on multimodal management of pancreatic ductal adenocarcinoma (PDAC). Created with BioRender (BioRender.com, accessed 10 September 2020). CT, computed tomography.
Figure 2
Figure 2
Sample collection, storage, DNA isolation, amplification and sequencing, and clinical application are important steps of ctDNA liquid biopsy. Created with BioRender (BioRender.com, accessed 10 September 2020). PCR, polymerase chain reaction.
See this image and copyright information in PMC

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