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. 2020 Oct 20;20(1):273.
doi: 10.1186/s12890-020-01281-w.

LncRNA SNHG10 is downregulated in non-small cell lung cancer and predicts poor survival

Meng Liang  1 , Linlin Wang  2 , Chuanhua Cao  3 , Shimao Song  1 , Feng Wu  4
Affiliations

LncRNA SNHG10 is downregulated in non-small cell lung cancer and predicts poor survival

Meng Liang et al. BMC Pulm Med. .

Abstract

Background: LncRNA SNHG10 has been reported to be an oncogenic lncRNA in liver cancer. However, its roles in non-small cell lung cancer (NSCLC) remains unknown.

Methods: Tumor and paired non-tumor tissues were harvested from 62 NSCLC patients. RT-qPCR was used to detect the expression of SNHG10 and miR-21 in tissues. Overexpression experiments were used to evaluate the interaction between SNHG10 and miR-21 in NSCLC cells. CCK-8 assay was used to detect the cell proliferation.

Results: We observed the expression of SNHG10 was down-regulated in non-small cell lung cancer (NSCLC) compared with that in non-tumor tissues. Moreover, we found that high expression levels of SNHG10 predicted favorable survival of NSCLC patients, and the expression of miR-21 were increased in NSCLC and inversely correlated with SNHG10 expression. In NSCLC cells, overexpression of SNHG10 resulted in increased miR-21 gene methylation and decreased miR-21 expression. Moreover, overexpression of SNHG10 attenuated the enhancing effect of miR-21 overexpression on cell proliferation.

Conclusions: SNHG10 may involve in NSCLC cell proliferation by regulating the miR-21 gene methylation.

Keywords: Methylation; NSCLC; SNHG10; miR-21.

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Conflict of interest statement

The authors declare that there is no conflict of interests.

Figures

Fig. 1
Fig. 1
Downregulation of SNHG10 is correlated with the poor survival of NSCLC patients. Expression of SNHG10 in paired tissues was determined by RT-qPCR. Levels of SNHG10 expression were compared between NSCLC and non-tumor tissues. Mean values were compared (a). ***, p < 0.001. To analyze survival, the 62 patients were divided into high and low SNHG10 level groups (n = 31, with median expression level of SNHG10 in NSCLC tissues as cutoff value). Survival curves were plotted and compared by log-rank test (b)
Fig. 2
Fig. 2
MiR-21 was upregulated in NSCLC and inversely correlated with SNHG10. Expression of miR-21 in paired NSCLC and non-tumor tissues from the 62 patients was determined by RT-qPCR. Levels of miR-21 expression were compared between NSCLC and non-tumor tissues. Mean values were compared (a). ***, p < 0.001. Linear regression was performed to analyze the correlations between SNHG10 and miR-21 across NSCLC tissues (b) and non-tumor tissues (c)
Fig. 3
Fig. 3
SNHG10 downregulated miR-21 in NSCLC cell through methylation. SNHG10 expression vector or miR-21 mimic was transfected into KLN 205 and HCC827 cells. Transfections were confirmed by RT-qPCR (a). The effects of SNHG10 overexpression on miR-21 (b), and the effects of miR-21 overexpression on SNHG10 (c) were also analyzed by RT-qPCR. MSP was performed to analyze the effects of SNHG10 overexpression on miR-21 (d). Mean ± SD values were presented and compared. M, methylation; U, un-methylation; *, p < 0.05.
Fig. 4
Fig. 4
SNHG10 overexpression attenuated the enhancing effect of miR-21 overexpression on cell proliferation. The roles of SNHG10 and miR-21 in regulating the proliferation of KLN 205 and HCC827 cells were analyzed by CCK-8. Mean ± SD values were presented and compared. *, p < 0.05
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