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. 2020 Oct 7:2020:4764219.
doi: 10.1155/2020/4764219. eCollection 2020.

Chemical Comparison of White Ginseng before and after Extrusion by UHPLC-Q-Orbitrap-MS/MS and Multivariate Statistical Analysis

Yun-Long Guo  1   2 , Yang Wang  2 , Yi-Lin Zhao  1 , Xiu-Ying Xu  1 , Hao Zhang  1 , Cheng-Bin Zhao  1 , Ming-Zhu Zheng  1 , Shu-Ying Liu  2 , Yu-Zhu Wu  1 , Jing-Sheng Liu  1
Affiliations

Chemical Comparison of White Ginseng before and after Extrusion by UHPLC-Q-Orbitrap-MS/MS and Multivariate Statistical Analysis

Yun-Long Guo et al. J Anal Methods Chem. .

Abstract

Ultrahigh-performance liquid chromatography Quadrupole-Orbitrap tandem mass spectrometry (UHPLC-Q-Orbitrap-MS/MS) was used to compare the composition of ginsenosides in white ginseng (WG) and extruded white ginseng (EWG). A total of 45 saponins, including original neutral ginsenosides, malonyl-ginsenosides, and chemical transformation of ginsenosides, were successfully identified in both WG and EWG. Multivariate statistical analyses including supervised orthogonal partial least squared discrimination analysis (OPLS-DA) and hierarchical clustering analysis (HCA) were used to analyze components of white ginseng before and after extrusion. As a result, three ginsenosides (malonyl (M)-Rb1, M-Rb2, and M-Rc) were found to be increased in WG, while three ginsenosides (Rb2, Rc, and Rg1) were elevated in EWG. In the OPLS-DA S-plot, the different compositions of ginsenoside that were distinguished between WG and EWG were screened out. Experimental results indicate that the UHPLC-Q-Orbitrap-MS/MS is a useful tool to characterize variations of ginsenosides in WG and EWG.

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Conflict of interest statement

The authors declare that they have no conflicts of interest.

Figures

Figure 1
Figure 1
Total ion chromatogram (TIC) of extruded white ginseng (a) and white ginseng (b).
Figure 2
Figure 2
The tandem mass spectrum of ginsenosides in the negative ion mode: (a) Rg1; (b) Rb1; (c) Ro; (d) mRb1.
Figure 3
Figure 3
The OPLS-DA/permutation test/S-plot of white ginseng and extruded white ginseng in negative ion mode.
Figure 4
Figure 4
The heat maps visualizing the intensities of the 17 ginsenoside datasets from white ginseng and extruded white ginseng (n = 10) and extruded white ginseng (n = 10).
See this image and copyright information in PMC

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