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. 2020 Aug;49(8):1494-1501.
doi: 10.18502/ijph.v49i8.3893.

The Expression and Diagnostic Value of LncRNA H19 in the Blood of Patients with Osteoarthritis

Liwu Zhou  1 , Yang Wan  1 , Qiang Cheng  1 , Ben Shi  1 , Lei Zhang  1 , Shuo Chen  1
Affiliations

The Expression and Diagnostic Value of LncRNA H19 in the Blood of Patients with Osteoarthritis

Liwu Zhou et al. Iran J Public Health. 2020 Aug.

Abstract

Background: To investigate the expression and diagnostic value of LncRNA H19 in the blood of patients with osteoarthritis.

Methods: A total of 130 cases of patients with osteoarthritis admitted to Jinling Hospital, Nanjing, China from Jun 2016 to Jul 2017 were elected as the study group, and 100 patients who underwent physical examination in Jinling Hospital during the same period were selected as the control group. The differences in expression levels of LncRNA H19 between the two groups were compared, the diagnostic value of LncRNA H19 in osteoarthritis and its relationship with clinical characteristics of patients with osteoarthritis were analyzed.

Results: The expression level of LncRNA H19 increased in peripheral blood of patients with osteoarthritis (P<0.05). The AUC, critical value, sensitivity and specificity of the diagnosis of osteoarthritis were 0.891, 1.879, 96.00% and 85.73%, respectively. The expression level of LncRNA H19 was related to K-L grading, and the expression level of LncRNA H19 increased with K-L grading. Pearson correlation analysis showed that LncRNA H19 was negatively correlated with bone metabolism indexes PINP, N-MID, BGP, BALP and Lysholm score (P<0.05), and positively correlated with bone metabolism indexes β-CTX, VAS score and WOMAC score (P<0.05).

Conclusion: LncRNA H19 is highly expressed in peripheral blood of patients with osteoarthritis, which is closely related to the occurrence and development of osteoarthritis and has a good diagnostic value for osteoarthritis.

Keywords: Blood; Diagnosis; Osteoarthritis.

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Conflict of interest statement

Conflict of interest The authors declare that there is no conflict of interest.

Figures

Fig. 1:
Fig. 1:
The expression level of LncRNA H19 in osteoarthritis The qRT-PCR results showed that the expression level of LncRNA H19 in peripheral blood of the study group was significantly higher than that of the control group. * means P<0.05
Fig. 2:
Fig. 2:
The diagnostic value of LncRNA H19 in osteoarthritis
Fig. 3:
Fig. 3:
Relationship between LncRNA H19 and clinical features of patients with osteoarthritis A: The relationship between LncRNA H19 and red blood cell count in patients with osteoarthritis. B: The relationship between LncRNA H19 and white blood cell count in patients with osteoarthritis. C: The relationship between LncRNA H19 and hemoglobin level in patients with osteoarthritis. D: The relationship between LncRNA H19 and PINP level in patients with osteoarthritis. E: The relationship between LncRNA H19 and β-CTX levels in patients with osteoarthritis. F: The relationship between LncRNA H19 and N-MID level in patients with osteoarthritis. G: The relationship between LncRNA H19 and BGP level in patients with osteoarthritis. H: The relationship between LncRNA H19 and BALP level in patients with osteoarthritis
Fig. 4:
Fig. 4:
Correlation analysis between LncRNA H19 and disease severity in patients with osteoarthritis A: The relationship between LncRNA H19 and VAS scores in patients with osteoarthritis. B: The relationship between LncRNA H19 and Lysholm score in patients with osteoarthritis. C: The relationship between LncRNA H19 and WOMAC score in patients with osteoarthritis
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