Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Oct 1:2020:4951251.
doi: 10.1155/2020/4951251. eCollection 2020.

Comprehensive Analysis of Differentially Expressed Circular RNAs in Patients with Senile Osteoporotic Vertebral Compression Fracture

Xiaocong Yao  1 , Minbo Liu  1 , Fang Jin  1 , Zhongxin Zhu  1   2
Affiliations

Comprehensive Analysis of Differentially Expressed Circular RNAs in Patients with Senile Osteoporotic Vertebral Compression Fracture

Xiaocong Yao et al. Biomed Res Int. .

Abstract

Aim: Circular RNAs (circRNAs) have been found to contribute to the regulation of many diseases and are abundantly expressed in various organisms. The present study is aimed at systematically characterizing the circRNA expression profiles in patients with senile osteoporotic vertebral compression fracture (OVCF) and predicting the potential functions of the regulatory networks correlated with these differentially expressed circRNAs.

Methods: The circRNA expression profile in patients with senile OVCF was explored by using RNA sequencing. The prediction of the enriched signaling pathways and circRNA-miRNA networks was conducted by bioinformatics analysis. Real-time quantitative PCR was used to validate the selected differentially expressed circRNAs from 20 patients with senile OVCF relative to 20 matched healthy controls.

Results: A total of 884 differentially expressed circRNAs were identified, of which 554 were upregulated and 330 were downregulated. The top 15 signaling pathways associated with these differentially expressed circRNAs were predicted. The result of qRT-PCR of the selected circRNAs was consistent with RNA sequencing.

Conclusions: CircRNAs are differentially expressed in patients with senile OVCF, which might contribute to the pathophysiological mechanism of senile osteoporosis.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
The qRT-PCR validation in the 20 paired blood samples from patients with senile OVCF compared with normal group and circRNA-miRNA-mRNA network prediction of circ_0079449 and circ_0122913. (a) The splicing junction sites of circ_0079449 and circ_0122913 were indicated by black arrow. (b) The relative expression of circ_0079449 and circ_0122913. (c) The top 5 miRNAs potentially regulated by circ_0079449 and circ_0122913, respectively, and top 5 target genes of each miRNA. OVCF, osteoporotic vertebral compression fracture.
Figure 2
Figure 2
Next-generation sequencing to identify the differences in expression profiles of circRNAs between the senile OVCF and control groups. (a, b) The volcano plot and scatter plot showed the dysregulated circRNAs. Green and red denote significantly downregulated circRNAs and upregulated circRNAs, respectively. (c) Hierarchical clustering heat map showes the circRNAs with altered expressions in senile OVCF samples compared with controls. Each column represents one sample and each row represents one circRNA. OVCF: osteoporotic vertebral compression fracture; circRNA: circular RNA.
Figure 3
Figure 3
GO, KEGG, and Reactome analyses of upregulated circRNAs. (a) Top 10 GO analysis to demonstrate the molecular functions of upregulated circRNAs (b) Top 15 significant enrichment KEGG pathways of upregulated circRNAs. (c) Top 15 significant enrichment Reactome pathways of upregulated circRNAs. GO: Gene Ontology; KEGG: Kyoto Encyclopedia of Genes and Genomes; circRNA: circular RNA.
Figure 4
Figure 4
GO, KEGG, and Reactome analyses of downregulated circRNAs. (a) Top 10 GO analysis to demonstrate the molecular functions of upregulated circRNAs (b) Top 15 significant enrichment KEGG pathways of downregulated circRNAs. (c) Top 15 significant enrichment Reactome pathways of downregulated circRNAs. GO: Gene Ontology; KEGG: Kyoto Encyclopedia of Genes and Genomes; circRNA: circular RNA.
Figure 5
Figure 5
The potential regulatory network of circRNA-miRNA interactions in peripheral blood from patients with senile OVCF. Red triangle node and blue triangle node represent upregulated and downregulated circRNAs,respectively. Blue circular node represents miRNAs. OVCF: osteoporotic vertebral compression fracture; circRNA: circular RNA; miRNA: microRNA.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Kanis J. A., McCloskey E. V., Johansson H., Oden A., Melton L. J., 3rd, Khaltaev N. A reference standard for the description of osteoporosis. Bone. 2008;42(3):467–475. doi: 10.1016/j.bone.2007.11.001. - DOI - PubMed
    1. Jacobs E., Senden R., McCrum C., van Rhijn L. W., Meijer K., Willems P. C. Effect of a semirigid thoracolumbar orthosis on gait and sagittal alignment in patients with an osteoporotic vertebral compression fracture. Clinical Interventions in Aging. 2019;14:671–680. doi: 10.2147/CIA.S199853. - DOI - PMC - PubMed
    1. Mohd-Tahir N. A., Li S. C. Economic burden of osteoporosis-related hip fracture in Asia: a systematic review. Osteoporosis International. 2017;28(7):2035–2044. doi: 10.1007/s00198-017-3985-4. - DOI - PubMed
    1. Center J. R. Fracture burden: what two and a half decades of Dubbo osteoporosis epidemiology study data reveal about clinical outcomes of osteoporosis. Current Osteoporosis Reports. 2017;15(2):88–95. doi: 10.1007/s11914-017-0352-5. - DOI - PubMed
    1. Xie Y., Gao Y., Zhang L., Chen Y., Ge W., Tang P. Involvement of serum-derived exosomes of elderly patients with bone loss in failure of bone remodeling via alteration of exosomal bone-related proteins. Aging Cell. 2018;17(3, article e12758) doi: 10.1111/acel.12758. - DOI - PMC - PubMed