Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Mar;33(2):279-282.
doi: 10.1177/1040638720967506. Epub 2020 Oct 21.

Detection of severe fever with thrombocytopenia syndrome virus and other viruses in cats via unbiased next-generation sequencing

Yasuyuki Momoi  1   2 , Aya Matsuu  3
Affiliations

Detection of severe fever with thrombocytopenia syndrome virus and other viruses in cats via unbiased next-generation sequencing

Yasuyuki Momoi et al. J Vet Diagn Invest. 2021 Mar.

Abstract

We used unbiased next-generation sequencing (NGS) to detect unknown viruses in cats. Serum or plasma samples were obtained from clinically ill cats with suspected acute viral infections. Nucleic acid was extracted from serum or plasma samples to construct a complementary DNA library for NGS. Comprehensive nucleotide sequencing analyses enabled detection of the genomes of various viruses, including the severe fever with thrombocytopenia syndrome virus, feline immunodeficiency virus, feline morbillivirus, parvovirus, and Torque teno felis virus. Our findings indicate that comprehensive nucleotide analyses of serum or plasma samples can be used to detect infections with unknown viruses in cats.

Keywords: cats; next-generation sequencing; severe fever with thrombocytopenia syndrome virus.

PubMed Disclaimer

Conflict of interest statement

Declaration of conflicting interests: The authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Altschul SF, et al.. Basic local alignment search tool. J Mol Biol 1990;215:403–410. - PubMed
    1. Benveniste RE, et al.. Segregation of RD-114 and FeLV-related sequences in crosses between domestic cat and leopard cat. Nature 1975;257:506–508. - PMC - PubMed
    1. Chandriani S, et al.. Identification of a previously undescribed divergent virus from the Flaviviridae family in an outbreak of equine serum hepatitis. Proc Natl Acad Sci USA 2013;110: 1407–1415. - PMC - PubMed
    1. De Luca E, et al.. A real-time RT-PCR assay for molecular identification and quantitation of feline morbillivirus RNA from biological specimens. J Virol Methods 2018;258:24–28. - PubMed
    1. Ewing B, et al.. Base-calling of automated sequencer traces using phred. I. Accuracy assessment. Genome Res 1998;8:175–185. - PubMed

MeSH terms

Supplementary concepts

LinkOut - more resources