Promoter selection by a bacterial enhancer-like activator element (BELE) in Escherichia coli

Abstract

The Escherichia coli glnA gene promoter glnAp2 is activated by an element able to act bidirectionally and at variable distance over the DNA. We demonstrate here that this activating element does not influence another promoter, 82p, adjacent to it, from which a gene is transcribed in opposite direction to glnA. Thus, although it displays a great flexibility, this element can activate selectively. The unresponsive promoter and glnAp2 are recognized by RNA polymerases complexed to two different sigma factors. Therefore, we argue that promoter selection by this element is dependent upon distinguishing the proper sigma factor.