Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Dec;57(12):4697-4706.
doi: 10.1007/s13197-020-04506-2. Epub 2020 May 9.

Development of an electrochemical biosensor for impedimetric detection of tetracycline in milk

Ayat Mohammad-Razdari  1 , Mahdi Ghasemi-Varnamkhasti  1   2 , Sajad Rostami  1 , Zahra Izadi  1   2 , Ali A Ensafi  3 , Maryam Siadat  4
Affiliations

Development of an electrochemical biosensor for impedimetric detection of tetracycline in milk

Ayat Mohammad-Razdari et al. J Food Sci Technol. 2020 Dec.

Abstract

Abstract: This study dealt with the fabrication of an impedimetric biosensor based on nanomaterial modified with pencil graphite electrode for the detection of tetracycline (TET) in milk samples. For response of the impedimetric aptasensor to be improved, the influence of different parameters (immobilization time of reduced grapheme oxide, time of aptamer, and TET binding, and concentration of aptamer) was optimized. In optimum conditions, the aptasensor provided a concentration range within 1 × 10-16 - 1 × 10-6 M and with a limit of detection of 3 × 10-17 M TET. The proposed impedimetric aptasensor was then used in milk samples analysis, and the acceptable recovery was achieved ranging from 92.8 to 102.1%. According to this study, the combination of an aptamer and electrochemical impedance spectroscopy is a promising method for detection of TET in milk samples with high reproducibility and stability.

Keywords: Antibiotics detection; Aptasensor; Electrochemical impedance spectroscopy; Nanocomposite; Reduced graphene oxide.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
A FTIR spectra of grapheme oxide (GO) and reduced grapheme oxide (RGO) and B SEM images of (a) modified PGE, (b) RGO/PGE, and (c) AuNPs/RGO/PGE
Fig. 2
Fig. 2
The CVs (a) and Nyquist plots (b) of different modified electrodes in 5.0 mM Fe(CN)64−/3− containing 0.1 M KCl, potential range of − 0.1 to 0.70 V with scan rate of 50 mV s−1, Conditions for EIS: the frequency range of 0.01 to 105 Hz at the potential of 150 mV
Fig. 3
Fig. 3
Influence of a immobilization time of RGO on the peak current, b immobilization time of aptamer, c aptamer concentration and d immobilization time of TET on the ∆Rct electrode in 5.0 mM Fe(CN)64−/3− containing 0.1 M KCl solution. The error bars represented the standard deviation (SD) (n = 3)
Fig. 4
Fig. 4
a calibration plot for ΔRct versus Log concentration of TET, b Nyquist diagram for different concentration of TET, (a-k) 1.0 × 10−16, 1.0 × 10−15, 1.0 × 10−14, 1.0 × 10−13, 1.0 × 10−12, 1.0 × 10−11, 1.0 × 10−10, 1.0 × 10−9, 1.0 × 10−8, 1.0 × 10−7, 1.0 × 10−6 M, respectively. In 5.0 mM Fe(CN)64−/3− containing 0.1 M KCl solution, The error bars represented the standard deviation (SD) (n = 3), c Selectivity of the fabricated aptasensor (n = 3) with streptomycin, penicillin G and sulfadiazine instead of TET, at the same concentrations (1 × 10–9 M). The error bars represented the SD (n = three electrodes)
See this image and copyright information in PMC

References

    1. Ayeni FA, Odumosu BT, Oluseyi AE, Ruppitsch W. Identification and prevalence of tetracycline resistance in enterococci isolated from poultry in Ilishan, Ogun State. Niger J Pharm Bioall Sci. 2016;8:69–73. doi: 10.4103/0975-7406.171729. - DOI - PMC - PubMed
    1. Benvidi A, Yazdanparast S, Rezaeinasab M, Tezerjani MD, Abbasi S. Designing and fabrication of a novel sensitive electrochemical aptasensor based on poly (L-glutamic acid)/MWCNTs modified glassy carbon electrode for determination of tetracycline. J Electroanal Chem. 2018;808:311–320. doi: 10.1016/j.jelechem.2017.12.032. - DOI
    1. Chen YN, Kong DZ, Liu LQ, Song SS, Kuang H, Xu CL. Development of an ELISA and immunochromatographic assay for tetracycline, oxytetracycline, and chlortetracycline residues in milk and honey based on the class-specific monoclonal antibody. Food Anal Methods. 2016;9:905–914. doi: 10.1007/s12161-015-0262-z. - DOI
    1. Da Silveira VG, Oliveira MS, de Almeida CA, Hoff RB, Mallmann CA. Liquid chromatography-tandem mass spectrometry determination and depletion profile of chlortetracycline, doxycycline, and oxytetracycline in broiler chicken muscle after oral administration. Food Anal Methods. 2018;11:2181–2194. doi: 10.1007/s12161-018-1199-9. - DOI
    1. De los Santos Álvarez N, Abedul MTF. Determination of ascorbic acid in dietary supplements by cyclic voltammetry. In: Teresa FernándezAbedul M, editor. Laboratory Methods in Dynamic Electroanalysis. Amsterdam: Elsevier; 2020. pp. 13–23.

LinkOut - more resources