Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Oct;26(10):2047-2052.
doi: 10.1007/s12298-020-00880-5. Epub 2020 Sep 15.

Natural rubber identification and characterization in Euphorbia macroclada

Affiliations

Natural rubber identification and characterization in Euphorbia macroclada

Somaye Azadi et al. Physiol Mol Biol Plants. 2020 Oct.

Abstract

Natural rubber is one of the most important polymers produced by plants, which contains cis-1,4-polyisoprene, protein and fatty acids. It has unique properties compared to synthetic rubber and has many different uses in industry. Here, natural rubber of Euphorbia macroclada was characterized due to its abundance in arid areas. Isolation of rubber was done using both acetone and hexane solvents. FT-IR and NMR further characterized and confirmed the structure of rubber as cis-1,4 polyisoprene. GPC analyses showed a molecular weight of 8.180E+2 with polydispersity of 1.287. These data is useful for better understanding of latex composition in family of Euphorbiaceae.

Keywords: E. macroclada; Latex; Natural rubber; Spurge.

PubMed Disclaimer

Conflict of interest statement

Conflict of interestThe authors declare that they have no conflict of interest.

Figures

Fig. 1
Fig. 1
Rubber extraction from E. macroclada latex. a Dualization of samples after adding solvent (acetone), b the extracted resin after evaporation of acetone. c The extracted rubber after drying hexane extract
Fig. 2
Fig. 2
Infrared spectroscopy of E. macroclada extracted rubber
Fig. 3
Fig. 3
1HNMR analysis of E. macroclada extracted rubber (left), cis-1,4-polyisoprene formula (right)
Fig. 4
Fig. 4
13CNMR analysis of E. macroclada extracted rubber
Fig. 5
Fig. 5
Molecular weight distribution of E. macroclada extracted rubber by GPC

References

    1. Amand LE, Tullin CJ. The theory behind FTIR analysis. Gothenburg: Department of Energy Conversion, Chalmers University of Technology; 1999.
    1. Arif SAM, Hamilton RG, Yusof F, Chew NP, Loke YH, Nimkar S, Beintema JJ, Yeang HY. Isolation and characterization of the early nodule-specific protein homologue (Hev b 13), an allergenic lipolytic esterase from Hevea brasiliensis latex. J Biol Chem. 2004;279(23):23933–23941. doi: 10.1074/jbc.M309800200. - DOI - PubMed
    1. Barla A, Bİrman H, KÜLTÜR Ş, Öksüz S. Secondary metabolites from Euphorbia helioscopia and their vasodepressor activity. Turk J Chem. 2006;30(3):325–332.
    1. Bell JL (2013) Biochemical and genetic characterization of rubber production in prickly lettuce (Lactuca serriola L.). Washington State University Ph.D Theses and Dissertations. Paper AAC3587052. 2013
    1. Bell JL, Burke IC, Neff MM. Genetic and biochemical evaluation of natural rubber from eastern Washington prickly lettuce (Lactuca serriola L.) J Agric Food Chem. 2015;63(2):593–602. doi: 10.1021/jf503934v. - DOI - PubMed

LinkOut - more resources