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. 2019 Nov-Dec;22(6):538-543.
doi: 10.4103/JCD.JCD_80_19. Epub 2020 Aug 20.

Chemical constituent, minimal inhibitory concentration, and antimicrobial efficiency of essential oil from oreganum vulgare against Enterococcus faecalis: An in vitro study

Krishnamachari Janani  1 , P Ajitha  1 , Raghu Sandhya  1 , Kavalipurapu Venkata Teja  1
Affiliations

Chemical constituent, minimal inhibitory concentration, and antimicrobial efficiency of essential oil from oreganum vulgare against Enterococcus faecalis: An in vitro study

Krishnamachari Janani et al. J Conserv Dent. 2019 Nov-Dec.

Abstract

Aim: The purpose of this study was to determine the chemical composition of oregano essential oil, minimal inhibitory concentration (MIC) and to assess its antimicrobial efficiency against Enterococcus faecalis.

Material and methods: Gas Chromatography and Mass Spectrometry (GC-MS) was used to determine the chemical composition of essential oil from oreganum vulgare. Broth dilution and agar diffusion method was used to evaluate the MIC. For Broth dilution, 100 μL of different concentration of oil (6.25, 12.5, 25.0, 50.0, and 100 μg/ml) was tested. Agar diffusion method was utilized to evaluate the antimicrobial efficiency of different concentration of oil (25.0, 50.0, and 100 μg/mL) against E. faecalis.

Results: GC-MS analysis revealed that oregano essential oil contained carvacrol (41.2%), γ-terpinene (12.68%), p-cymene (9.47%), α-terpinene (1.19%) as the major compounds and β-caryophyllene (0.83%), β-linalool (0.67%), β-bisabolene (0.601%), α-pinene (0.6%), β-pinene (0.5%), terpinen-4-ol (0.41%), borneol (0.4%), 3-thujene (0.4%), spathulenol (0.4%), myristicin (0.25%), and apiol (0.14%). The results of the present study reported Oregano essential oil possess antimicrobial activity against E. faecalis. The MIC was 25 μg/ml and the minimum bacterial concentration (MBC) was 50 μg/ml.

Conclusion: Oregano essential oil was reported to be an effective antimicrobial agent against E. faecalis. The MIC was found to be 25 μg/ml and the MBC was found to be 50 μg/ml.

Keywords: Antimicrobial efficiency; minimal inhibitory concentration; oregano essential oil; oreganum vulgare.

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Conflict of interest statement

There are no conflicts of interest.

Figures

Figure 1
Figure 1
Broth dilution method
Figure 2
Figure 2
Zone of inhibition
See this image and copyright information in PMC

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