Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Nov-Dec;22(6):564-567.
doi: 10.4103/JCD.JCD_242_19. Epub 2020 Aug 20.

In vitro comparative evaluation of antifungal efficacy of three endodontic sealers with and without incorporation of chitosan nanoparticles against Candida albicans

Satabdi Pattanaik  1 , Amit Jena  2 , Govind Shashirekha  1
Affiliations

In vitro comparative evaluation of antifungal efficacy of three endodontic sealers with and without incorporation of chitosan nanoparticles against Candida albicans

Satabdi Pattanaik et al. J Conserv Dent. 2019 Nov-Dec.

Abstract

Aim: The aim of this study was to compare the antifungal efficacy of three endodontic sealers (AH Plus, Apexit Plus, and MTA Fillapex) with and without the incorporation of chitosan nanoparticles against Candida albicans.

Materials and methods: The present study was carried out by the Kirby-Bauer method. C. albicans were cultured in Sabouraud Dextrose Agar plates. Filter papers (n = 10) were placed in the cultured Petri dishes and the sealers were mixed according to the manufacturer's instructions and placed on the top of the filter papers. Group division of sealers is as follows: Group I - AH Plus, Group II - Apexit Plus, and Group III - MTA Fillapex. Group IC, Group IIC, and Group IIIC were the addition of 2% chitosan nanoparticles with respective sealers. Plates were incubated for 18 h, and the zone of inhibition was measured with a measuring scale and values (in millimeter) were recorded. Statistical analysis was done by one-way analysis of variance followed by post hoc multiple pair-wise comparisons.

Results: All the tested groups showed statistically significant difference (P < 0.05) from each other. Two percent chitosan-incorporated groups showed superior zone of inhibition compared to sealers used alone. Group IC (16.35 ± 0.71 mm) had the highest zone of inhibition followed by Group I (13.8 ± 0.86 mm). For the remaining groups, the zone of inhibition was in the following order: Group IIC > Group II > Group IIIC > Group III.

Conclusion: AH Plus sealer mixed with 2% chitosan showed significantly higher antifungal property. Mixing of 2% chitosan with endodontic sealer provides an added advantage so that endodontic re-infections can be minimized and will be helpful in retreatment cases.

Keywords: Antifungal activity; Candida albicans; chitosan; endodontic sealers.

PubMed Disclaimer

Conflict of interest statement

There are no conflicts of interest.

Similar articles

See all similar articles

Cited by

References

    1. Gomes BP, Pedroso JA, Jacinto RC, Vianna ME, Ferraz CC, Zaia AA, et al. In vitro evaluation of the antimicrobial activity of five root canal sealers. Braz Dent J. 2004;15:30–5. - PubMed
    1. Song M, Kim HC, Lee W, Kim E. Analysis of the cause of failure in nonsurgical endodontic treatment by microscopic inspection during endodontic microsurgery. J Endod. 2011;37:1516–9. - PubMed
    1. Ashraf H, Samiee M, Eslami G, Ghodse Hosseini MR. Presence of Candida Albicans in root canal system of teeth requiring endodontic retreatment with and without periapical lesions. Iran Endod J. 2007;2:24–8. - PMC - PubMed
    1. Endo MS, Ferraz CC, Zaia AA, Almeida JF, Gomes BP. Quantitative and qualitative analysis of microorganisms in root-filled teeth with persistent infection: Monitoring of the endodontic retreatment. Eur J Dent. 2013;7:302–9. - PMC - PubMed
    1. Bouillaguet S, Wataha JC, Tay FR, Brackett MG, Lockwood PE. Initial in vitro biological response to contemporary endodontic sealers. J Endod. 2006;32:989–92. - PubMed