Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Sep 5;29(11):1563-1571.
doi: 10.1007/s10068-020-00820-9. eCollection 2020 Nov.

Antioxidant activity and calcium bioaccessibility of Moringa oleifera leaf hydrolysate, as a potential calcium supplement in food

Ye-Rang Yun  1 , Su-Jin Oh  1 , Min-Jung Lee  1 , Yun-Jung Choi  1 , Sung Jin Park  1 , Mi-Ai Lee  1 , Sung-Gi Min  1 , Hye-Young Seo  2 , Sung-Hee Park  1
Affiliations

Antioxidant activity and calcium bioaccessibility of Moringa oleifera leaf hydrolysate, as a potential calcium supplement in food

Ye-Rang Yun et al. Food Sci Biotechnol. .

Abstract

Moringa oleifera leaf (ML) is rich in vitamins and minerals, specially abundant calcium, therefore it is widely used as a calcium supplement for food. This study aimed to investigate the antioxidant activity and calcium bioaccessibility of M. oleifera leaf hydrolysate (MLH) as a calcium supplement for kimchi. MLH was prepared under three different proteases, two different protease contents, and three different incubation times. Total phenol content (TPC), total flavonoid content (TFC), and antioxidant activities were investigated. Cellular activity and calcium bioaccessibility were also investigated. The highest calcium level of MLH was observed in 3% Protamex treatment for 4 h. TPC, TFC, and antioxidant activities of MLH in Protamex and Alcalase treatments were higher than those in Flavourzyme treatment (p < 0.05). Moreover, high cell viability and alkaline phosphatase activity were also observed in C2C12 cells. Kimchi containing MLH showed high calcium accessibility compared to kimchi alone. Taken together, the application of MLH could have potential as a calcium supplement for kimchi production.

Keywords: Alkaline phosphatase activity; Antioxidant activity; Bioaccessibility; Calcium; Moringa oleifera leaf hydrolysate; Protease.

PubMed Disclaimer

Conflict of interest statement

Conflict of interestAll authors declare no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Figures

Fig. 1
Fig. 1
Scheme of experimental design in this study
Fig. 2
Fig. 2
Cell cytotoxicity and alkaline phosphatase (ALP) activity of MLH on C2C12 cells. (A) Cytotoxicity and (B) ALP activity. Data are expressed as mean ± SD. Different lowercase letters indicate significant differences among groups (p < 0.05)
See this image and copyright information in PMC

References

    1. Abdull Razis AF, Ibrahim MD, Kntayya SB. Health benefits of Moringa oleifera. Asian Pac. J. Cancer P. 2014;15:8571–8576. doi: 10.7314/APJCP.2014.15.20.8571. - DOI - PubMed
    1. Ademiluyi AO, Aladeselu OH, Oboh G, Boligon A. Drying alters the phenolic constituents, antioxidant properties, α-amylase, and α-glucosidase inhibitory properties of Moringa (Moringa oleifera) leaf. Food Sci. Nutr. 2018;6:2123–2133. doi: 10.1002/fsn3.770. - DOI - PMC - PubMed
    1. Adetola OY, Kruger J, White Z, Taylor JRN. Comparison between food-to-food fortification of pearl millet porridge with moringa leaves and baobab fruit and with adding ascorbic and citric acid on iron, zinc and other mineral bioaccessibility. LWT Food Sci. Technol. 2019;106:92–97. doi: 10.1016/j.lwt.2019.02.044. - DOI
    1. Allen JC, Issa JY and Cai W. Calcium content, in vitro digestibility, and bioaccessibility in leaves of spinach (Spinacia oleracea), sweet potato (Ipomea batatas), and drumstick tree (Moringa oleifera). F1000Res. 3: 65 (2019)
    1. Apak R, Özyürek M, Güçlü K and Çapanoğlu E. Antioxidant activity/capacity measurement. 1. Classification, physicochemical principles, mechanisms, and electron transfer (ET)-based assays. J. Agric. Food Chem. 64: 997-1027 (2016) - PubMed

LinkOut - more resources