Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Dec;103(6):2350-2352.
doi: 10.4269/ajtmh.20-1079. Epub 2020 Oct 23.

A Sensitive Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Direct Visual Detection of SARS-CoV-2

Yee Ling Lau  1 , Ilyiana Binti Ismail  2 , Nur Izati Binti Mustapa  2 , Meng Yee Lai  1 , Tuan Suhaila Tuan Soh  2 , Afifah Haji Hassan  2 , Kalaiarasu M Peariasamy  3 , Yee Leng Lee  3 , Pik Pin Goh  4
Affiliations

A Sensitive Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Direct Visual Detection of SARS-CoV-2

Yee Ling Lau et al. Am J Trop Med Hyg. 2020 Dec.

Abstract

A simple and rapid reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the detection of SARS-CoV-2. The RT-LAMP assay was highly specific for SARS-CoV-2 and was able to detect one copy of transcribed SARS-CoV-2 RNA within 24 minutes. Assay validation performed using 50 positive and 32 negative clinical samples showed 100% sensitivity and specificity. The RT-LAMP would be valuable for clinical diagnosis and epidemiological surveillance of SARS-CoV-2 infection in resource-limited areas as it does not require the use of sophisticated and costly equipment.

PubMed Disclaimer

Figures

Figure 1.
Figure 1.
Detection of loop-mediated isothermal amplification (LAMP) products by the color change method using hydroxy naphthol blue. (A) The negative LAMP reaction remained in violet color (B), whereas the positive reaction was indicated by a color change from violet to sky blue.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. World Health Organization , 2020a. WHO Health Emergency Dashboard. Geneva, Switzerland: WHO; Available at: https://extranet.who.int/publicemergency. Accessed August 18, 2020.
    1. World Health Organization , 2020b. Novel Coronavirus (2019-nCoV) Technical Guidance: Laboratory Testing for 2019-nCoV in Humans. Geneva, Switzerland: WHO; Available at: https://www.fda.gov/media/134922/download. Accessed August 18, 2020.
    1. Pham HM, Nakajima C, Ohashi K, Onuma M, 2005. Loop-mediated isothermal amplification for rapid detection of Newcastle Disease Virus. J Clin Microbiol 43: 1646–1650. - PMC - PubMed
    1. Chen HT, Zhang J, Sun D, Ma L, Liu XT, Cai XP, Liu YS, 2008. Development of reverse transcription loop-mediated isothermal amplification for rapid detection of H9 avian influenza virus. J Virol Methods 151: 200–203. - PubMed
    1. Lau YL, et al. 2020. Real-time reverse transcription loop-mediated isothermal amplification for rapid detection of SARS-CoV-2. Peer J 8: e9278. - PMC - PubMed

MeSH terms

Supplementary concepts