Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2021 Mar;99(3):243-250.
doi: 10.1002/cyto.a.24246. Epub 2020 Nov 11.

Comprehensive Phenotyping of Peripheral Blood T Lymphocytes in Healthy Mice

Genny Del Zotto  1 , Elisa Principi  2 , Francesca Antonini  1 , Serena Baratto  2 , Chiara Panicucci  2 , Claudio Bruno  2 , Lizzia Raffaghello  2
Affiliations
Free article
Review

Comprehensive Phenotyping of Peripheral Blood T Lymphocytes in Healthy Mice

Genny Del Zotto et al. Cytometry A. 2021 Mar.
Free article

Abstract

T lymphocytes play a central role in antigen-specific immune responses. They modulate the function of different immune cells both through a direct contact (receptor binding) and through the secretion of cytokines. At the same time, they are deeply involved in the direct killing of aberrant target cells. T lymphocytes derive from a bone marrow precursor that migrates in the thymus where the main differentiation steps take place. Mature CD4 and CD8 single-positive cells, then, leave the thymus to reach the secondary lymphoid organs. T-cell subsets and their maturation steps can be identified mainly based on the expression of extracellular markers, intracellular transcription factors and cytokine production profiles. In this review, we report, from a cytometric point of view, an overview of the most important T-cell subpopulations and their differentiation state. © 2020 International Society for Advancement of Cytometry.

Keywords: CD4; CD8: T-regulatory cells; flow cytometry; mouse T lymphocytes; phenotyping.

PubMed Disclaimer

References

Literature Cited

    1. Cho JH, Sprent J. TCR tuning of T cell subsets. Immunol Rev 2018;283(1):129-137.
    1. Egawa T. Regulation of CD4 and CD8 coreceptor expression and CD4 versus CD8 lineage decisions. Adv Immunol 2015;125:1-40.
    1. Klein L, Hinterberger M, Wirnsberger G, Kyewski B. Antigen presentation in the thymus for positive selection and central tolerance induction. Nat Rev Immunol 2009;9(12):833-844.
    1. Buus TB, Jee MH, Ødum N. OMIP-057: Mouse γδ T-cell development characterize by a 14 color flow Cytometry panel. Cytometry A 2019;95(7):726-729.
    1. Treiner E, Lantz O. CD1d- and MR1-restricted invariant T cells: Of mice and men. Curr Opin Immunol 2006;18(5):519-526.

Publication types

LinkOut - more resources