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. 1987 Oct;84(19):6810-4.
doi: 10.1073/pnas.84.19.6810.

Construction of a yeast strain devoid of mitochondrial introns and its use to screen nuclear genes involved in mitochondrial splicing

B Séraphin  1 , A Boulet, M Simon, G Faye
Affiliations

Construction of a yeast strain devoid of mitochondrial introns and its use to screen nuclear genes involved in mitochondrial splicing

B Séraphin et al. Proc Natl Acad Sci U S A. 1987 Oct.

Abstract

We have constructed a respiring yeast strain devoid of mitochondrial introns to screen nuclear pet- mutants for those that play a direct role in mitochondrial intron excision. Intron-less mitochondria are introduced by cytoduction into pet- strains that have been made rho0; cytoductants therefrom recover respiratory competency if the original pet- mutation is required only for mitochondrial splicing. By this means, we have identified 11 complementation groups of such genes. Their total number may be estimated as about 18.

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