Corrigendum: Akt2 Regulates the Differentiation and Function of NKT17 Cells via FoxO-1-ICOS Axis

Abstract

[This corrects the article DOI: 10.3389/fimmu.2018.01940.].

Keywords: Akt2; FoxO-1; ICOS; NKT17; iNKT.

Figure 3

Loss of Akt2 reduces the proliferation and apoptosis of iNKT cells. (A) Flow cytometry of Ki-67 cells gated on iNKT cells in the thymus. (B,C) Percentage of Ki-67 cells in the thymus and spleen (n = 3 mice per group). (D) Flow cytometry of Ki-67 cells gated on NKT17 cells in the thymus. (E,F) Percentage of Ki-67 cells gated on NKT17 cells in the thymus (n = 3 mice per group) and spleen (n = 3 mice per group). (G) Flow cytometry of Annexin V cells gated on the iNKT in the thymus. (H,I) Percentage of Annexin V cells in the thymus and spleen of WT (n = 3), KO (n = 3), WT chimera mice (n = 4) and Akt2 KO chimera mice (n = 3). (J) Flow cytometry of Annexin V cells gated on the stage2 (CD24⁻CD44⁺NK1.1⁻) iNKT cells in the thymus of WT, KO, and chimera mice. (K,L) Percentage of Annexin V cells of stage2 iNKT cells in the thymus and spleen of WT (n = 3), KO (n = 3), WT chimera mice (n = 4), and Akt2 KO chimera mice (n = 3). (M) Flow cytometry of CD1d cells gated on 7AAD cells in the thymus. (N,O) Percentage of CD1d cells in the thymus (n = 3 mice per group) and spleen (n = 3 mice per group). (P) Flow cytometry of Bcl2 cells gated on iNKT cells in the thymus. (Q,R) Overlaid histograms show expression of Bcl2.chimerachimera *p < 0.05; **p < 0.01, Student's t-test.

Figure 4

Akt2 regulates the NKT17 differentiation by promoting the expression of ICOS. (A–F) The percentages of ICOS of WT and Akt2 KO cells in thymus (n = 3 mice per group) and spleen (n = 3 mice per group) is shown. (C,F) Overlaid histograms show expression of ICOS in the thymus and spleen. (G–L) The percentages of ICOS of CD45.2⁺ cells in thymus and spleen of WT chimera mice (n = 4) and Akt2 KO chimera mice (n = 3) is shown. (I,L) Overlaid histograms show expression of ICOS in the thymus and spleen of chimeras. (M,N) The Flow cytometry of c-Maf and expression of c-Maf in the thymus. (O,P) Flow cytometry of IL-23R and expression of IL-23R in the thymus. (Q–S) mRNA levels of indicated molecules in WT and Akt2 KO iNKT cells. Expression of indicated mRNA from MACS- and FACS- sorted WT and Akt2 KO iNKT cells from freshly isolated thymocytes was quantified by real-time qPCR from three independent experiments. *p < 0.05; **p < 0.01, Student's t-test. (T) Flow cytometry analysis of the pSTAT3 for MFI in thymocytes from WT (n = 3) and Akt2 KO mice (n = 3). (U) Overlaid histograms show expression of pSTAT3 after α-GalCer stimulated 72 h. (V) Airway response to methacholine of WT (n = 3) and Akt2 KO mice (n = 3). LR, lung resistance from two independent experiments. *p < 0.05, Student's t-test.