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. 2021 Feb;7(1):40-48.
doi: 10.1002/cre2.337. Epub 2020 Oct 25.

Cytotoxicity evaluation of dental and orthodontic light-cured composite resins

Raoul Bationo  1   2 , Ablassé Rouamba  3 , Abdoulaziz Diarra  4 , Monique Lydie Ahia Beugré-Kouassi  2   5 , Jean-Bertin Beugré  2   5 , Fabienne Jordana  6
Affiliations

Cytotoxicity evaluation of dental and orthodontic light-cured composite resins

Raoul Bationo et al. Clin Exp Dent Res. 2021 Feb.

Abstract

Introduction: The aim of this study was to determine the cytotoxicity of light-cured composite resins (Clearfil ES-2, Clearfil ES Flow, Filtek Supreme XTE, Grengloo, Blugloo, Transbond XT, and Transbond LR) then to assess leachable components in contact with human gingival fibroblasts (GFs) and to quantity detected bisphenol A (BPA).

Methods: Light-cured composite resin discs were immersed for 24 hours in gingival fibroblastic medium (n = 3 for each product) and in control medium (n = 2 for each product) contained in plate. Cytotoxicity of the products (n = 95) was determined by the measure of cell viability using MTT assay after reading the optical densities of the plates. The analysis of leachable components was done by gas phase chromatography and mass spectrometry (GC-MS) and detected BPA was quantified. The limit of quantification was 0.01 μg/mL. Statistical analyses were performed by using IBM SPSS Statistics 20 and Kruskal-Wallis and Mann-Whitney U-tests were applied.

Results: Cell viabilities were between 85 and 90%. Many chemical compounds including triethylene glycol dimethacrylate (TEGDMA) and BPA were identified. The average concentrations were 0.67 μg/mL ± 0.84 in the control medium and 0.73 μg/mL ± 1.05 in the fibroblastic medium. Filtek Supreme XTE presented the highest concentration of BPA with 2.16 μg/mL ± 0.65 and Clearfil ES Flow presented the lowest with 0.25 μg/mL ± 0.35. No BPA was detected with Transbond XT and Transbond LR. Clearfil ES Flow, Filtek Supreme XTE, Grengloo and Transbond LR presented residual TEGDMA.

Conclusions: Light-cured composite resins are slightly cytotoxic opposite GFs and release many components including BPA and TEGDMA. Clinical precautions should be taken to decrease the release of these monomers.

Keywords: GC-MS; cytotoxicity; light-cured composite resin; monomers.

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Conflict of interest statement

The authors declare no potential conflicts of interest.

Figures

FIGURE 1
FIGURE 1
Cell viability
FIGURE 2
FIGURE 2
Spectrum and concentration of BPA detected with Blugloo immersed in the fibroblastic medium. BPA, bisphenol A
FIGURE 3
FIGURE 3
Mass spectrum showing TEGDMA detected with Transbond LR immersed in the fibroblastic medium. TEGDMA, triethylene glycol dimethacrylate
See this image and copyright information in PMC

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