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Published Erratum
. 2020 Nov 10;117(45):28531-28533.
doi: 10.1073/pnas.2019643117. Epub 2020 Oct 26.

Correction for Bos et al., Phytophthora infestans effector AVR3a is essential for virulence and manipulates plant immunity by stabilizing host E3 ligase CMPG1

No authors listed
Published Erratum

Correction for Bos et al., Phytophthora infestans effector AVR3a is essential for virulence and manipulates plant immunity by stabilizing host E3 ligase CMPG1

No authors listed. Proc Natl Acad Sci U S A. .
No abstract available

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Figures

Fig. 1.
Fig. 1.
AVR3aKI and AVR3aEM interact with and stabilize CMPG1. (A) AVR3aKI and AVR3aEM with (21-147) and without (60-147; shown only for AVR3aKI) RXLR-encoding portions interact with CMPG1 in Y2H (LacZ and -His reporter genes activated). Whereas AVR3aKI/Y147del (Y147 deletion) and AVR3aKI/Y147S mutants fail to interact with CMPG1, the AVR3aKI/Y147F mutant interacts. (B) Western blots probed with anti-myc and anti-HA antibodies following expression of myc-CMPG1 in binding domain vector, with HA-AVR3aEM (21-147), HA-AVR3aKI (21-147), HA-AVR3aKI/Y147del (60-146) activation domain (AD) fusions, or AD alone, in yeast (each indicated with a white star). Full-length immunoblots are shown in Fig. S8. (C) Western blots probed with anti-myc and anti-FLAG antibodies, showing a time-course of transient coexpression (by agroinfiltration) of FLAG-AVR3aKI, FLAG-AVR3aEM, FLAG- AVR3aKI/Y147del or a vector control with 4x-myc-ΔN-StCMPG1a at 2 and 3 d postinoculation (dpi). (D) As in C, but at 5 dpi. (E) As in C but at 5 dpi with 4x-myc-StCMPG1b (full-length) and 4x-myc-ΔN-StCMPG1b (lacking the N-terminal 29 amino acids). Protein sizes are indicated in kDa. Protein loading is shown by Coomassie blue (CBB) or Ponceau S (PS) staining. (F) Fluorimeter measurements (in relative fluorescence units) following coexpression in N. benthamiana of the split YFP constructs CMPG1::N-YFP (YN), CMPG1::C-YFP (YC), C-YFP::AVR3aKI, C-YFP::AVR3aEM, C-YFP::AVR3aKI/Y147del as indicated. (G) Confocal microscopy following coexpression in N. benthamiana of split YFP constructs CMPG1-YC with a vector expressing free N-YFP, and CMPG1-YC with N-YFP::AVR3aKI, N-YFP::AVR3aEM, and N-YFP::AVR3aKI/Y147del constructs as indicated in the panels. (Scale bars, 200 μm.)
Fig. S8.
Fig. S8.
Full length immunoblots used for the revised Fig. 1B showing proteins revealed by anti-myc or anti-HA antibodies as indicated, with corresponding Ponceau stains (PS) shown below each immunoblot.
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