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. 1987 Jun;163(2):391-7.
doi: 10.1016/0003-2697(87)90240-5.

Transformation of yeast spheroplasts without cell fusion

P M Burgers  1 , K J Percival
Affiliations

Transformation of yeast spheroplasts without cell fusion

P M Burgers et al. Anal Biochem. 1987 Jun.

Abstract

The efficiency of genetic transformation of Saccharomyces cerevisiae spheroplasts has been increased 10- to 100-fold over previously published procedures. Optimal transformation frequencies for single-stranded and double-stranded replicating plasmids are 2 X 10(7) and 5 X 10(6) transformants/microgram, respectively. At saturating DNA concentrations, 12 and 3%, respectively, of the viable spheroplasts contain plasmid DNA. The percentage of transformants that have undergone nuclear fusion varies from 0.1 to 3%, indicating that fusion is not required for the uptake of DNA by yeast spheroplasts.

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