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. 2020 Dec;64(12):815-824.
doi: 10.1111/1348-0421.12857. Epub 2020 Dec 1.

Detection rates of periodontal bacteria and herpesviruses in different forms of periodontal disease

Miljan Puletic  1 , Branka Popovic  2 , Sasa Jankovic  1 , Gavrilo Brajovic  3
Affiliations
Free article

Detection rates of periodontal bacteria and herpesviruses in different forms of periodontal disease

Miljan Puletic et al. Microbiol Immunol. 2020 Dec.
Free article

Abstract

The aim was to investigate the detection rates of periodontal bacteria (Porphyromonas gingivalis, Tannerella forsythia, Prevotella intermedia, and Aggregatibacter actinomycetemcomitans) and herpesviruses (herpes simplex virus-1 [HSV-1], cytomegalovirus [CMV], and Epstein-Barr virus [EBV]) in different forms and severity of periodontal disease, and to compare them with those in periodontally healthy subjects. One hundred and twenty-nine patients participated in the study: 39 diagnosed with periodontal abscess (PA), 33 with necrotizing ulcerative periodontitis (NUP), 27 with chronic periodontitis (CP), and 30 participants with healthy periodontal tissue represented a healthy control group. All patients with periodontal disease (PA, NUP, and CP) were also divided into two groups according to the severity of their disease: moderate and severe periodontitis. The subgingival samples were collected from the periodontitis active sites and the detection of microorganisms was performed by end-point polymerase chain reaction analyses. The results revealed significantly higher detection rates of P. gingivalis, T. forsythia, and P. intermedia in all three groups of patients with periodontitis than in healthy participants. The highest detection rate of A. actinomycetemcomitans was noticed in CP, which was significantly higher than that in PA, NUP, and healthy control. The occurrence of EBV was significantly higher in NUP than in CP and healthy participants. CMV was detected significantly more frequently in PA and NUP than in CP and healthy participants. Comparisons among healthy participants and patients with moderate and severe periodontitis showed significantly higher detection rates of EBV and CMV in patients with severe forms of periodontitis than in healthy participants and those with moderate periodontitis.

Keywords: Herpesviridae; chronic periodontitis; necrotizing ulcerative periodontitis; periodontal abscess; polymerase chain reaction.

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References

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