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. 2020 Aug 25;9(8):3965-3969.
doi: 10.4103/jfmpc.jfmpc_523_20. eCollection 2020 Aug.

Association of specific microorganisms with endodontic signs and symptoms. A comparative study

Kishore Kumar Singh  1 , Pankaj Kumar  2 , Pragyan Das  3 , Manjula Marandi  4 , Swagat Panda  5 , Amit Mahajan  6 , Dinesh Kumar  7
Affiliations

Association of specific microorganisms with endodontic signs and symptoms. A comparative study

Kishore Kumar Singh et al. J Family Med Prim Care. .

Abstract

Aim: The study aimed to evaluate the association of root canal microorganisms red complex and E. facealis with endodontic clinical signs and symptoms using polymerase chain reaction.

Materials and methods: Bacterial samples were obtained using sterile paper points from the teeth of 100 subjects divided into two groups; Group I: 50 individuals with primary tooth infections and Group II: 50 individuals with failed endodontic treatment having the secondary infection. DNA extracted from samples was analyzed for endodontic pathogens by using species-specific primers.

Results: The pain was noticed in 66%, (33 of 50 subjects) in primary infection and 60% (30 of 50) in a secondary infection. A statistically significant association between pain and E. faecalis bacteria observed both in primary infection and secondary infection (P < 0.05). Tenderness on percussion was associated with 40% cases in Group I and 70% cases in Group II. The red complex accounted for 94% of cases associated with tenderness on percussion in primary infection while 86% of cases associated with secondary infection with a statistically significant association (P < 0.05).

Conclusion: Prevalence of red complex bacteria and E. faecalis suggested the association of studied bacteria with symptomatic infected pulp and periradicular diseases.

Keywords: Bacteria; pulp; red complex.

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Conflict of interest statement

There are no conflicts of interest.

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