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. 2020 Jun 3;1(1):100014.
doi: 10.1016/j.xpro.2020.100014. eCollection 2020 Jun 19.

Chromatin-Bound Proteome Profiling by Genome Capture

Sergi Aranda  1 , Eva Borràs  1   2 , Eduard Sabidó  1   2 , Luciano Di Croce  1   2   3
Affiliations

Chromatin-Bound Proteome Profiling by Genome Capture

Sergi Aranda et al. STAR Protoc. .

Abstract

De novo identification of chromatin interactors can reveal unexpected pathways relevant to physiology and human disease. Inspired by the DNA mediated chromatin pull-down (Dm-ChP) technology (also known as iPOND [isolation of proteins on nascent DNA]) for the proteomic characterization of nascent DNA, we have recently reported a new experimental protocol that allows for the identification of proteins on total DNA (iPOTD) for bulk chromatome profiling and de novo identification of chromatin-bound proteins. Here, we detail a step-by-step protocol to survey the cellular chromatin-bound proteome in a simple, robust, and unbiased manner. For complete details on the use and execution of this protocol, please refer to Aranda et al. (2019).

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Conflict of interest statement

The authors declare no competing interests.

Figures

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Graphical abstract
Figure 1
Figure 1
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References

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