Prevalence of Human Polyomavirus JC and BK in Normal Population

Abstract

JC virus (JCV) , and BK virus (BKV) can remain latency in kidney and excrete via urine asymptomatically. JCV has been associated with colorectal and bladder cancers. BKV has been linked with lung, pancreas, liver, urogenital tract, head and neck cancers. Therefore, the frequency of JCV DNA and BKV DNA are essential to evaluate in urine samples of healthy individuals.

Materials and methods: Hundred sixty four urine samples were collected from healthy subjects [96 females and 68 males]. DNA was extracted and detection of JCV DNA and BKV DNA was carried out by PCR . The analysis of sequencing and construction of phylogenetic tree were performed for the samples positive for JCV DNA and BKV DNA.

Results: Ten (6.09%) urine samples [5/96(5.2%) females and 5/68( 8.82) males] were tested positive for JCV DNA (P= 0.814). The results of sequencing and phylogenetic tree showed the isolated JCV DNA were cluster with 3A genotype. 21/164 (12.8%) samples were tested positive for BKV DNA [11/96(11.45%) females and males 10/68(14.7%)] ( P= 0.63). The results of sequencing and phylogenetic tree showed that the isolated BKV was cluster with genotype III.

Conclusion: In the present study 6.09% and 12.8% of the healthy individuals showed positive for JCV DNA (genotype 3A) and BKV DNA(genotype III) respectively. With regard to life threating diseases by BKV and JCV in immunocomprsied patients , the screening BKV DNA and JCV DNA should be implemented for patients with cancer /autoimmune diseases /organ recipient/ multiple sclerosis (MS), prior to immunosuppression therapy or immunomodulatory agents treatment.<br />.

Keywords: BK Virus; JC Virus; Polymerase Chain Reaction.

Figure 1

Phylogenetic Tree was Constructed by Neighbor Joining Method for VP1 Region of the BKV DNA Isolated from a Urine Sample. The isolated BKV DNA (MN480315) from Iran was compared with other different isolated BKVs genotypes (I-IV) retrived from Genebank. The isolated BKV (MN480315) in the black circle was cluster with BKV genotype III USA (KF055893.1). The constructed neighbor joining method is under Kimura two-parameter model by 1000 bootstrap replicates. The scale Bar =0.01

Figure 2

Phylogenetic Tree was Constructed by Neighbor Joining Method for VP1 Region of the Isolated JCV in Ahvaz city, Iran. The two isolated JCV (MN480316 and MN480317). with black squar were compared with different JCVs genotypes(1-4) and subtypes isolated from different regions of the world. The two isolated JCVs were cluster with JCV genotype III Tanzania (U73500). The constructed neighbor joining method is under Kimura two-parameter model by 1000 bootstrap replicates The scale Bar =0.005