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Review
. 2020 Oct 8:8:815.
doi: 10.3389/fcell.2020.00815. eCollection 2020.

Neural Stem Cells and Methods for Their Generation From Induced Pluripotent Stem Cells in vitro

Adelya A Galiakberova  1   2 , Erdem B Dashinimaev  2   3
Affiliations
Review

Neural Stem Cells and Methods for Their Generation From Induced Pluripotent Stem Cells in vitro

Adelya A Galiakberova et al. Front Cell Dev Biol. .

Abstract

Neural stem cells (NSCs) provide promising approaches for investigating embryonic neurogenesis, modeling of the pathogenesis of diseases of the central nervous system, and for designing drug-screening systems. Such cells also have an application in regenerative medicine. The most convenient and acceptable source of NSCs is pluripotent stem cells (embryonic stem cells or induced pluripotent stem cells). However, there are many different protocols for the induction and differentiation of NSCs, and these result in a wide range of neural cell types. This review is intended to summarize the knowledge accumulated, to date, by workers in this field. It should be particularly useful for researchers who are beginning investigations in this area of cell biology.

Keywords: ESC; NSC; iPSC; neural stem cells; neurogenesis; pluripotent stem cells; regenerative medicine.

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Figures

FIGURE 1
FIGURE 1
A simplified presentation of the general principles of neural induction. Neural induction of PSCs may be conducted in 2D conditions (monolayer and coculture) or in 3D conditions (embryoid bodies). At the same time, further generation and/or cultivation of NSCs can be achieved in 2D monoculture and neural rosettes or in 3D cultures (SFEB and SFEBq, brain organoids, neurospheres). PSCs, pluripotent stem cells; NSCs, neural stem cells; EB, embryoid body; SFEB(q), (quick) serum free culture of embryoid bodies; [№] are references to examples of the protocols presented in Tables 1, 2.
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