Marginal adaptation, solubility and biocompatibility of TheraCal LC compared with MTA-angelus and biodentine as a furcation perforation repair material

Abstract

Background: This study evaluated the marginal adaptation, solubility and biocompatibility of TheraCal LC compared with mineral trioxide aggregate (MTA-Angelus) and Biodentine when used as a furcation perforation repair material.

Methods: The marginal adaptation was assessed by scanning electronic microscope and presence of any gap between the dentin surface and filling material in each quadrant of the sample was analyzed at 1000 X magnification. The solubility was measured after one week by the ISO standard method. Biocompatibility was evaluated by the inflammatory response and radiography after one month and three months of repair of experimental furcation perforations in dog's teeth.

Results: There were significant differences in the marginal adaptation, solubility and biocompatibility of the tested materials (P < 0.05). TheraCal LC showed the highest frequency distribution of gap presence that was followed by the MTA-Angelus then Biodentine. The least soluble material after one week was TheraCal LC that was followed by the MTA-Angelus and Biodentine. After one month and three months, TheraCal LC showed the highest inflammatory response and highest frequency distribution of radiolucency that was followed by the Biodentine then MTA-Angelus.

Conclusion: Unlike Biodentine, TheraCal LC is incapable of alternating the MTA in furcation perforation repair due to its poor biocompatibility and poor marginal adaptation.

Keywords: Biocompatibility; Biodentine; Furcal perforation; MTA; Marginal adaptation; TheraCal LC.

Fig. 1

SEM photomicrographs (magnification X1000) of cavities filled with MTA-angelus (a, b), Biodentine (c, d) and TheraCal LC (e, f) showing no gap evident between the materials and dentin (a, c, e) and a gap evident between the materials and dentin (b, d, f)

Fig. 2

a A representative periapical radiograph of the MTA subgroup immediately post perforation repair. b Periapical radiograph of the MTA subgroup showing absence of furcal radiolucency and bony defect after 3 months of evaluation. c A representative periapical radiograph of the Biodentine subgroup immediately post perforation repair. d A periapical radiograph of the Biodentine subgroup showing absence of furcal radiolucency and bony defect after 3 months of evaluation

Fig. 3

a A representative periapical radiograph of the TheraCal LC subgroup immediately post perforation repair. b A periapical radiograph of the TheraCal LC subgroup showing presence of furcal radiolucency and bony defect after 3 months of evaluation. c A representative periapical radiograph of the positive control subgroup immediately post perforation. d A periapical radiograph of the positive control subgroup showing presence of furcal radiolucency and bony defect after one month of evaluation

Fig. 4

a A representative photomicrograph of the MTA-Angelus subgroup showing a score 1 inflammatory cell count after 1 month of evaluation (H&E, × 400). b A representative photomicrograph of the Biodentine subgroup showing a score 2 inflammatory cell count after 1 month of evaluation (H&E, × 400). c A representative photomicrograph of the TheraCal LC subgroup showing a score 3 inflammatory cell count after three months of evaluation (H&E, × 400). d A representative photomicrograph of the positive control subgroup showing a score 3 inflammatory cell count after three months of evaluation (H&E, × 400)