Mapping of the constitutive lysyl-tRNA synthetase gene of Escherichia coli K-12
- PMID: 3312173
- PMCID: PMC213943
- DOI: 10.1128/jb.169.11.5311-5313.1987
Mapping of the constitutive lysyl-tRNA synthetase gene of Escherichia coli K-12
Abstract
The constitutive lysyl-tRNA synthetase gene (lysS) was mapped at 62.1 min on the Escherichia coli chromosome by a combination of conjugation and transduction, with physical confirmation by two-dimensional gel electrophoresis. Revertant analysis suggests that the altered isoelectric point and the low amount of the mutant LysS protein may be due to a single mutational event.
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