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. 2020 Oct 30;72(6):855-872.
doi: 10.1007/s10616-020-00430-7. Online ahead of print.

The comparison of miRNAs that respond to anti-breast cancer drugs and usnic acid for the treatment of breast cancer

Demet Cansaran-Duman  1 , Ümmügülsüm Tanman  2 , Sevcan Yangın  2 , Orhan Atakol  3
Affiliations

The comparison of miRNAs that respond to anti-breast cancer drugs and usnic acid for the treatment of breast cancer

Demet Cansaran-Duman et al. Cytotechnology. .

Abstract

This study was designed to compare usnic acid with anti-breast cancer drug molecules (A-BCDM) routinely used in the treatment of breast cancer. The miRNA information of 17 anti-breast cancer drug used in breast cancer treatment was obtained from the Small Molecule-miRNA Network-Based Inferance (SMIR-NBI) tool. We had been determined common and different expressed miRNAs between 17 A-BCDM & usnic acid and were classified according to the common miRNAs to reveal molecular similarity. As a result of the bioinformatic analyzes, 20 common miRNAs were determined between 17 A-BCDM and usnic acid. The common miRNAs were analyzed with bioinformatic tolls for determining pathways and targets. The most common miRNAs for 6 of 17 A-BCDM and usnic acid were determined as miR-374a-5p and miR-26a-5p. We compared the anti-proliferative effect of usnic acid and one of the 17 A-BCDM that tamoxifen on MDA-MB-231 triple negative breast cancer cell with real-time cell analysis system. The real time PCR assay was carried out with miR-26a-5p for evaluate to expression level of MDA-MB-231 breast cancer cell and MCF-12A non-cancerous epithelial breast cell. As a result of study, usnic acid as novel candidate drug molecule showed high similarity ratio with 5-Fluorouracil, Sulindac Sulfide, Curcumin and Cisplatin A-BCDM used in treatment of breast cancer. miR-26a-5p as common response miRNA of usnic acid and tamoxifen was showed a decreased level of expression by validated qRT-PCR assay. The obtained from study, in addition to 17 A-BCDM, usnic acid has also the potential to be used as a candidate molecule in the treatment of breast cancer. Moreover, miR-26a-5p might be used as a biomarker in the treatment of breast cancer but further analysis is required.

Keywords: Anti-breast cancer drugs; Breast cancer; Usnic acid; miRNA.

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Conflict of interest statement

The authors declare that they have no conflict of interest.

Figures

Fig. 1
Fig. 1
miRNA numbers and similarity rates commonly expressed between usnic acid and 17 anti-breast cancer drugs
Fig. 2
Fig. 2
The similarity relationship between miRNAs responding to the usnic acid and the miRNAs responding to the anti-breast cancer drugs
Fig. 3
Fig. 3
miRNAs of 17 anti-cancer drugs and usnic acid with similar expression levels (*: The common miRNA profiles of 17 anti-cancer drugs and usnic acid
Fig. 4
Fig. 4
The number of target gene of common miRNAs between usnic acid and 17 anti-breast cancer drugs
Fig. 5
Fig. 5
The determination of antiproliferative effect for tamoxifen and usnic acid by XCELLigence assay
Fig. 6
Fig. 6
The expression analyses for miR-26a-5p on MDA-MB-231 and MCF-12A cell lines treated with usnic acid by usnig qRT-PCR assay. (*p < 0.01) (**p < 0.001)
Fig. 7
Fig. 7
Antiproliferative effect of miR-26a-5p on MDA-MB-231 (green = control, red = scramble, blue:miR-26a-5p). Each transfection is quantified in duplicate (p < 0.001). (Color figure online)
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References

    1. Bartel D. MicroRNAs. Cell. 2004;116:281–297. doi: 10.1016/S0092-8674(04)00045-5. - DOI - PubMed
    1. Comprehensive molecular portraits of human breast tumours (2012) Nature 490:61–70 - PMC - PubMed
    1. Creighton C, Nagaraja A, Hanash S, Matzuk M, Gunaratne P. A bioinformatics tool for linking gene expression profiling results with public databases of microRNA target predictions. RNA. 2008;14:2290–2296. - PMC - PubMed
    1. Dent R, Trudeau M, Pritchard K, Hanna W, Kahn H, Sawka C, Lickley L, Rawlinson E, Sun P, Narod S. Triple-negative breast cancer: clinical features and patterns of recurrence. Clin Cancer Res. 2007;13:4429–4434. - PubMed
    1. Dinçsoy A, Cansaran Duman D. Changes in apoptosis-related gene expression profiles in cancer cell lines exposed to usnic acid lichen secondary metabolite. Turk J Biol. 2017;41:484–493.