Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Mar 15;148(6):1489-1498.
doi: 10.1002/ijc.33370. Epub 2020 Nov 9.

Short tandem repeat profiling for the authentication of cancer stem-like cells

Paola Visconti  1 , Federica Parodi  1 , Barbara Parodi  1 , Lucia Casarino  2 , Paolo Romano  3 , Mariachiara Buccarelli  4 , Roberto Pallini  5 , Quintino Giorgio D'Alessandris  5 , Andrea Montori  6 , Emanuela Pilozzi  6 , Lucia Ricci-Vitiani  4
Affiliations

Short tandem repeat profiling for the authentication of cancer stem-like cells

Paola Visconti et al. Int J Cancer. .

Abstract

Colorectal and glioblastoma cancer stem-like cells (CSCs) are essential for translational research. Cell line authentication by short tandem repeat (STR) profiling ensures reproducibility of results in oncology research. This technique enables to identify mislabeling or cross-contamination of cell lines. In our study, we provide a reference dataset for a panel of colorectal and glioblastoma CSCs that allows authentication. Each cell line was entered into the cell Line Integrated Molecular Authentication database 2.1 to be compared to the STR profiles of 4485 tumor cell lines. This article also provides clinical data of patients from whom CSCs arose and data on the parent tumor stage and mutations. STR profiles and information of our CSCs are also available in the Cellosaurus database (ExPASy) as identified by unique research resource identifier codes.

Keywords: cell line authentication; colorectal tumor; glioblastoma; human stem-like cell lines; short tandem repeat profiling.

PubMed Disclaimer

Conflict of interest statement

The authors declared no potential conflicts of interest.

References

    1. Grande S, Palma A, Ricci‐Vitiani L, et al. Metabolic heterogeneity evidenced by MRS among patient‐derived Glioblastoma Multiforme stem‐like cells accounts for cell clustering and different responses to drugs. Stem Cells Int. 2018;2018:3292704. - PMC - PubMed
    1. Marziali G, Buccarelli M, Giuliani A, et al. A three‐microRNA signature identifies two subtypes of glioblastoma patients with differentclinicaloutcomes. Mol Oncol. 2017;11:1115‐1129. - PMC - PubMed
    1. D'Alessandris QG, Biffoni M, Martini M, et al. The clinical value of patient‐derived glioblastoma tumorspheres in predicting treatment response. Neuro Oncol. 2017;19:1097‐1108. - PMC - PubMed
    1. Pallini R, Ricci‐Vitiani L, Banna GL, et al. Cancer stem cell analysis and clinical outcome in patients with glioblastoma multiforme. Clin Cancer Res. 2008;14:8205‐8212. - PubMed
    1. Ricci‐Vitiani L, Lombardi DG, Pilozzi E, et al. Identification and expansion of human colon‐cancer‐initiating cells. Nature. 2007;445:111‐115. - PubMed

Publication types