doi: 10.1007/BF00495386.
Electroncytochemical and biochemical demonstration of guanylate cyclase activity in the pancreatic islet
- PMID: 33129
- DOI: 10.1007/BF00495386
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Electroncytochemical and biochemical demonstration of guanylate cyclase activity in the pancreatic islet
Histochemistry.
.
Abstract
With a cytochemical method using guanylyl imidodiphosphate as a substrate, the guanylate cyclase activity was localized on the plasma membrane of A, B and D cells of islets of Langerhans isolated from the rat. Adequate control experiments were performed by a double-blind method. Parallel biochemical assay showed that guanylate cyclase activity was not completely lost after fixation with 1% glutaraldehyde and incubation with 4 mM lead nitrate. Furthermore, the depressed activity was still stimulatable with acetylcholine.
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