Differential responses of thiol metabolism and genes involved in arsenic detoxification in tolerant and sensitive genotypes of bioenergy crop Ricinus communis

Abstract

Castor, a non-food, dedicated bioenergy crop, has immense potential to be used for phytoremediation/revegetation of heavy metal contaminated sites. In the previous study, we identified arsenate [As(V)]-tolerant (WM) and As(V)-sensitive (GCH 2) genotypes of castor (Ricinus communis L.) with differential accumulation and tolerance of arsenic [As]. The role of thiols in As(V) toxicity and tolerance mechanism in the castor plant is not fully understood. On the one hand, thiol-dependent reduction of As(V) to As(III) by arsenate reductase (AR) makes it capable of reacting with thiol groups of protein leading to disturbed metabolic pathways; on the other hand, reduction of As(V) to arsenite [As(III)] by AR and then complexation of As(III) with phytochelatins (PCs) and compartmentalization of As(III)-PC complex are considered as the major detoxification mechanisms of As(V). In our study, the expression of RcAR increased in leaves and roots of As(V)-tolerant castor genotype WM but decreased in sensitive genotype GCH 2 due to 200 μM As(V) treatment. The activity of glutathione reductase (GR) increased significantly in the tolerant genotype, whereas it remained same in the sensitive genotype. GSH/GSSH ratio declined substantially in the sensitive genotype. The increased expression of phytochelatin synthase 1 isoform 1 (RcPCS1X1) in roots, RcPCS1X2 and metallothionein type 2 (RcMT2) in leaves, and c-type ABC transporter (RcABCC) in roots and leaves of WM was observed, but the expression of these genes declined or remained the same in GCH 2. Overall, our results suggest the essential roles of GR, RcAR, RcPCS1, RcMT2, and RcABCC in the tolerance of WM castor genotype to As(V) toxicity.

Keywords: ABCC transporter; Arsenate; Castor; Glutathione reductase; Metallothionein; Phytochelatin synthase.