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Case Reports
. 2020 Nov;8(11):1154-1158.
doi: 10.1016/S2213-2600(20)30418-5. Epub 2020 Sep 24.

Human recombinant soluble ACE2 in severe COVID-19

Alexander Zoufaly  1 , Marko Poglitsch  2 , Judith H Aberle  3 , Wolfgang Hoepler  4 , Tamara Seitz  4 , Marianna Traugott  4 , Alexander Grieb  4 , Erich Pawelka  4 , Hermann Laferl  4 , Christoph Wenisch  4 , Stephanie Neuhold  4 , Doris Haider  5 , Karin Stiasny  3 , Andreas Bergthaler  6 , Elisabeth Puchhammer-Stoeckl  3 , Ali Mirazimi  7 , Nuria Montserrat  8 , Haibo Zhang  9 , Arthur S Slutsky  9 , Josef M Penninger  10
Affiliations
Case Reports

Human recombinant soluble ACE2 in severe COVID-19

Alexander Zoufaly et al. Lancet Respir Med. 2020 Nov.

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Figures

Figure 1
Figure 1
Human recombinant soluble ACE2 pharmacodynamics and drug concentrations (A) Renin–angiotensin system fingerprinting of angiotensin metabolites in relation to human recombinant soluble ACE2 dosing. Circle sizes and angiotensin values correspond to metabolite concentrations (pmol/L). Black arrows mark the catalysing reactions mediated by the indicated enzymes. (B) Plasma concentrations of various ACE2 substrates and catalysed products. (C) ACE2 activity as the sum of ACE2 products and downstream metabolites related to plasma renin activity. (D) ACE2 activity (angiotensin II-to-angiotensin 1–7 catalytic conversion assays) in plasma and tracheal suctions. First dose administered on day 0; red triangles indicate days on which drug was administered. ACE2=angiotensin converting enzyme 2. AT1R=angiotensin II type 1 receptor. MAS=G protein coupled receptor Mas. NEP=neutral endopeptidase.
Figure 2
Figure 2
Inflammatory, endothelial, and alveolar biomarkers (A) Plasma concentrations of IL-6, IL-8, sRAGE, and ferritin. (B) Plasma concentrations of C-reactive protein, TNFα, surfactant protein D, and angiopoietin-2. Red triangles indicate days on which drug was administered. IL=interleukin. sRAGE=soluble advanced glycation end product receptor. TNFα=tumour necrosis factor.
Figure 3
Figure 3
Viral load and neutralising antibodies Viral load (blue circles) and neutralising antibodies (green squares) in plasma (A) and tracheal suctions and nasopharyngeal swabs (B). PCR LOD was 100 copies per mL. Red triangles indicate days on which drug was administered. LOD=limit of detection.
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References

    1. Bourgonje AR, Abdulle AE, Timens W. Angiotensin-converting enzyme 2 (ACE2), SARS-CoV-2 and the pathophysiology of coronavirus disease 2019 (COVID-19) J Pathol. 2020;251:228–248. - PMC - PubMed
    1. Zhang H, Penninger JM, Li Y, Zhong N, Slutsky AS. Angiotensin-converting enzyme 2 (ACE2) as a SARS-CoV-2 receptor: molecular mechanisms and potential therapeutic target. Intensive Care Med. 2020;46:586–590. - PMC - PubMed
    1. Monteil V, Kwon H, Prado P. Inhibition of SARS-CoV-2 infections in engineered human tissues using clinical-grade soluble human ACE2. Cell. 2020;181:905–913. - PMC - PubMed
    1. Kuba K, Imai Y, Rao S. A crucial role of angiotensin converting enzyme 2 (ACE2) in SARS coronavirus-induced lung injury. Nat Med. 2005;11:875–879. - PMC - PubMed
    1. Crackower MA, Sarao R, Oudit GY. Angiotensin-converting enzyme 2 is an essential regulator of heart function. Nature. 2002;417:822–828. - PubMed

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